B P International

The present work aimed the prospection of microorganisms from Bauhinia monandra leaves, with the purpose to identify endophytics to obtain strains with possible biotechnological applications. B. monandra leaves, disinfected with hypochlorite solution, were macerated in phosphate buffered saline and seeded in ten culture media containing antibacterial or antifungal agents. The endophytic filamentous fungus strains detected belonged to the genera Penicillium, Curvullaria and Aspergillus. Non-filamentous endophyte bacteria were grouped in the genera Bacillus, Burkholderia, Enterobacter and strains of endophytic Actinobacteria were classified as Streptomyces and Nocardiopsis. The isolation of endophytic microorganisms with nine culture media revealed better bacteria development with L-arginine agar; inorganic salt starch agar and potato dextrose agar were superior to Actinobacteria and fungus strains, respectively. The present study revealed the predominance of the genus Penicillium in ...

The Actinobacteria receive much attention, since they produce a wide variety of metabolites, including antibiotics, antitumor agents, antioxidant molecules and enzyme inhibitors. These bacteria can be found in various habitats, including soil, ocean, extreme environments, mangrove, lichen, plants, and animals. The classification of Actinobacteria based upon the morphological observation, physiological and biochemical characteristics is not enough to differentiate the genera of this phylum. Following, a complementary identification is performed based on the distribution of specific constituents of the cellular wall, such as diaminopimelic acid and carbohydrates. With the advent of molecular biology, the identification of genera and species became more reliable. The screening of microbial natural products has become an important route to discover new bioactive compounds in order to develop new therapeutic agents. Actinobacteria remains one of the leading producers of biopharmaceutical...