Protease Composition in Tissue Extracts of Hydrobionts from Antarctic Region: Recent Study | Chapter 13 | Current Research Trends in Biological Science Vol. 1
Aims: Marine hydrobionts, which grow in extreme conditions, e.g. low temperatures, are an important source of enzymes with unique properties. By this reason the proteases from cold-water organisms could have a considerable biotechnological and therefore, commercial significance. The objective of the current study was to investigate the proteolytic potential of marine hydrobionts from Antarctic region (an example of Odontaster validus and Glyptonotus antarcticus). Methodology: SDS-PAGE was carried out for the determination of protein composition in extracts. The proteolytic activity was monitored by zymographic technique. Further, the samples were preincubated with protease inhibitors EDTA, PMSF and SBTI and then total proteolytic (with casein as substrate) activity was measured. Gel filtration chromatography was applied for the fractionation of tested extracts. Collagenolytic and trypsin-like (amidase activity) activities were assessed with help of native collagen type I and L-B...