Histological Studies on Heteracris littoralis (Rambur) Treated with Silica Nano-particles/ Challenger Formulation | Chapter 13 | Recent Advances in Biological Research Vol. 5
Aim: Testing Local diatomaceous earth (Silica nano-particles) and commercial pesticide (Challenger 36% SC) formulation against alfalfa grasshopper, Heteracris littoralis (Rambur, 1838) (Orthopetra: Acrididae) under laboratory conditions. Study Design: Histological study for alimentary tract using light microscope. Place and Duration of Study: Department of Pests and Plant Protection, National Research Centre, Egypt- 2016-2017. Methodology: Fourth instar nymphs of H. littoralis were taken from laboratory culture reared on semi-artificial diet [1] for the experiments. The nymphs were fed on diet mixed with 1% concentration of Silica nano-particles/Challenger formulation. The tissue specimens of the alimentary canal were dissected in 0.9% NaCl solution and fixed in Bouin's solution for 24 hours [2] then dehydrated in ascending alcoholic series and cleared in Xylen for few seconds, and then specimens were infiltrated in three changes of paraffin wax each last...