AYA and HANA: New Seedless Mandarin’s Triploid Hybrids Selected in Morocco | Chapter 05 | Advances and Trends in Biotechnology and Genetics Vol. 1
Seedlessness is a major objective in Morocco Citrus improvement programme. Triploidy has played an important role in the development of new seedless mandarin cultivars for fresh fruit market and has received increasing attention for the consumers. Citrus triploid hybrids can be recovered through 2x x 2x taking advantage of the unreduced (2n) gametes formation. 2x x 4x and 4x x 2x sexual hybridizations are also widely exploited. In that case, many series of diploid crosses were assessed. At maturity, the fruits have been harvested, and small embryos were extracted from undeveloped seed and cultured on the medium of Murashig and Skoog supplemented with 1mg/l gibberellic acid according. Triploids seedling selected by flow cytometry analysis were budded on Troyer citrange and planted in INRA domain with spacing 6 m X 3 m. Two new seedless selections of mandarins have been evaluated in a citrus breeding program at Morocco INRA: Hana and Aya that were compared with their female parent cle...