B P International

Aims: The present study was undertaken to explore the potentiality of in vitro micropropagation (by callus induction) of Ipomoea obscura(L.) Ker-Gawl (Convolvulaceae), a perennial herb. Method: Axenically grown cotyledonary leaves regenerated into profuse calli, in various combinations of naphthaleneacetic acid (NAA), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and 2,4dichlorophenoxyacetic acid (2,4 D) with benzylaminopurine (BAP). Microroots emerged from these calli when subjected to various combinations of NAA and BAP. Maximum number of root meristems and microroots were formed in 1.25 mg/L NAA with the combination of 0.5 mg/L BAP (1:4 v/v). Leaf, nodal and internodal segments as explants were cultured on MS media containing 2,4 D at 0.5, 1.25, 2.50, 5.0 and 10.0 mg/L, for callus induction. Only leaf tips initiated callogenesis with highest response at 10 mg/l 2,4 D and lowest with 0.5 mg/l 2.4 D. This is the first report of in vitro response of I. obscura.   Resul...

Aims: The present study was undertaken to explore the potentiality of in vitro micropropagation (by callus induction) of Ipomoea obscura(L.) Ker-Gawl (Convolvulaceae), a perennial herb. Method: Axenically grown cotyledonary leaves regenerated into profuse calli, in various combinations of naphthaleneacetic acid (NAA), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and 2,4dichlorophenoxyacetic acid (2,4 D) with benzylaminopurine (BAP). Microroots emerged from these calli when subjected to various combinations of NAA and BAP. Maximum number of root meristems and microroots were formed in 1.25 mg/L NAA with the combination of 0.5 mg/L BAP (1:4 v/v). Leaf, nodal and internodal segments as explants were cultured on MS media containing 2,4 D at 0.5, 1.25, 2.50, 5.0 and 10.0 mg/L, for callus induction. Only leaf tips initiated callogenesis with highest response at 10 mg/l 2,4 D and lowest with 0.5 mg/l 2.4 D. This is the first report of in vitro response of I. obscura.   Resul...

Simmondsia chinensis (Link) Schneider (Simmondsiaceae) is a evergreen shrub of desert and marginal land of India. It produces a unique liquid-wax which has commercial potential in pharmaceutical industry. It is difficult to propagate vegetatively as it is a seasonal procedure and grows slowly. Therefore, there is a need to improve the propagation method through seed germination and seedling behaviour. The present investigation was carried out to assess viability and in vitro germination tests of fresh and old seeds. It was found that light (16/8-h day/night photoperiod) conditions resulted in early and higher seed germination percentage as compared to total dark period under in vitro culture conditions. Improvement in germination percentage of one-year-old seeds was observed using Gibberellic acid (GA 3 ), Benzylaminopurine (BAP) and Thidiazuron (TDZ) under given photoperiod conditions. Interestingly, TDZ 1.0 mg/L -1 were produced highest germination rate of seeds (92%), as well as...

Sida cordifolia Linn. finds frequent mention in Ayurvedic System of Medicine for many therapeutic properties including beneficial effect upon male reproductive processes. Aim of present in vitro study was to evaluate impact of aqueous extract of Sida cordifolia roots on rat spermatozoa in terms of count, motility & morphology and through HOS study following standard assessment methods to assess any likely reproductive toxicity, spermicidal action, lethality or abnormalities in animal experimentation. Sexually mature (18-22 weeks old) male Wister rats weighing 180 to 260 gm were used. Sperms were collected from cauda epididymis of rat testes and sperm suspension was prepared by mixing them with 2 ml of 5% Sucrose at 37°C, dividing into 4 samples – control (A) and 3 drug treated samples (B, C & D) containing 10, 20 or 30 mg/ml of aqueous extract of Sida cordifolia. The various sperm parameters were observed under Olympus CX41 microscope. Sperm concentration, Progressive and No...