Investigating Proteomic Variations in Transgenic Tomatoes Compared to Its Non-transgenic Counterpart | Chapter 13 | Modern Research in Botany Vol. 1
Three complementary approaches were used for the assessment of proteomic variations due to genetic transformation. These approaches were gel electrophoresis, Gas chromatography coupled with mass spectrometry (GC/MS) and amino acids analysis. First we assessed proteomic variations applying gel electrophoresis analysis in two different transformed plants (GM-potato and GM-Tomato) along with their non-transformed counterparts. Further we pursued with our analysis using one of the tested GM-plants (transgenic tomato) and its non-transgenic counterpart applying Gas chromatography coupled with mass spectrometry (GC/MS) and amino acids analysis. The results indicated proteomic variations between both transgenic plants (GM-potato and GM-tomato) compared to their non-transgenic counterparts, where the protein patterns separation on the 1D SDS-PAGE were not similar in both cases. Results of the amino acid concentrations of the transformed tomato were also different compared to its non-tra...