Phospholipase A2 Inhibition and Antiinflammatory Activity of F4 Fraction of Total Ethereal Leaf Extract of Annona senegalensis Pers. (Annonaceae) | Chapter 02 | New Insights on Chemical Research Vol. 1
Annona senegalensis Pers. (Annonaceae) is a plant which is used in african traditional medicine for the treatment of various diseases. This study aimed to investigate the analgesic and anti-inflammatory activity of total ethereal leaf extract fractions of A. senegalensis. Compounds of methanolic fractions of ethereal leaf extract of A. senegalensis were separated by gel sephadex chromatography, in five fractions (F1, F2, F3, F4, F5). Experiments were performed in acetic acid-induced contortions in mice, carrageenan rat paw edema and phospholipase A2 inhibitory test. The methanolic fraction of total ethereal leaf extract (10 mg/kg, per os) significantly prevented the carrageenan inflammatory edema. The variation of edema is 22.31 ± 3.35%, 49.66 ± 13.50%, 52.10 ± 10 .02% respectively at T1h, T3h and T5h. The increased edema after oral administration of F4 fraction administered at 300 µg/kg and 1 mg/kg per os is respectively 52.77 ± 7.36% and 33.81 ± 6.94%. The variation of edema in be...