B P International

Aims: The present study was undertaken to explore the potentiality of in vitro micropropagation (by callus induction) of Ipomoea obscura(L.) Ker-Gawl (Convolvulaceae), a perennial herb. Method: Axenically grown cotyledonary leaves regenerated into profuse calli, in various combinations of naphthaleneacetic acid (NAA), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and 2,4dichlorophenoxyacetic acid (2,4 D) with benzylaminopurine (BAP). Microroots emerged from these calli when subjected to various combinations of NAA and BAP. Maximum number of root meristems and microroots were formed in 1.25 mg/L NAA with the combination of 0.5 mg/L BAP (1:4 v/v). Leaf, nodal and internodal segments as explants were cultured on MS media containing 2,4 D at 0.5, 1.25, 2.50, 5.0 and 10.0 mg/L, for callus induction. Only leaf tips initiated callogenesis with highest response at 10 mg/l 2,4 D and lowest with 0.5 mg/l 2.4 D. This is the first report of in vitro response of I. obscura.   Resul...

Aims: The present study was undertaken to explore the potentiality of in vitro micropropagation (by callus induction) of Ipomoea obscura(L.) Ker-Gawl (Convolvulaceae), a perennial herb. Method: Axenically grown cotyledonary leaves regenerated into profuse calli, in various combinations of naphthaleneacetic acid (NAA), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and 2,4dichlorophenoxyacetic acid (2,4 D) with benzylaminopurine (BAP). Microroots emerged from these calli when subjected to various combinations of NAA and BAP. Maximum number of root meristems and microroots were formed in 1.25 mg/L NAA with the combination of 0.5 mg/L BAP (1:4 v/v). Leaf, nodal and internodal segments as explants were cultured on MS media containing 2,4 D at 0.5, 1.25, 2.50, 5.0 and 10.0 mg/L, for callus induction. Only leaf tips initiated callogenesis with highest response at 10 mg/l 2,4 D and lowest with 0.5 mg/l 2.4 D. This is the first report of in vitro response of I. obscura.   Resul...

The morphological characterization and micro propagation were carried out on Aloe vera (Aloe barbadensis Miller) accessions collected from different locations in India were discussed in this book chapter. The experiments were conducted on 12 Aloe accessions and the leaf length, width, thickness, stem length and thickness were measured using verniercalipers. The plantlets were chemically treated with 2.5% (w/v) of sodium hypochlorite which is an alternative safer surface sterilization method for replacement of mercury chloride by using shoot tip as explants. The sterilization results indicate that the highest number (93%) of viable explants (10) with the best regeneration potential was in Murashige and Skoog (MS) medium compared with other treatments. Multiple shoots/explants (35) were found in MS medium containing 6-benzylaminopurine (BAP, 1.5 mgL -1 ; kinetin (0.5 mgL -1 ), indole-3-butyric acid (IBA, 0.2 mg L- 1 ), indole-3-acetic acid (IAA, 0.1 mg L -1 ), and naphthalene acetic a...