Micropropagation of Narcissus tazetta ‘Chinensis’ and Its Relation to Secondary Metabolites Content: Brief Overview | Chapter 5 | Current Research Trends in Biological Science Vol. 1
In this study, bulbs of Narcissus tazetta ‘Chinensis’ were used as explants for micropropagation. Several disinfection protocols, callus medium and regeneration medium were tested in addition to the secondary metabolites content analysis. Four disinfection protocols were used. The most successful was: 1) disinfection of bulbs in 70% ethanol and 2% HgCl2, 10 minutes for each, outside cabinet, or 2) 70% ethyl alcohol (4 min), 20% clorox (5 min), 0.1% mercuric chloride (1 min) and 9% hydrogen peroxide (1 min) inside cabinet, washing with sterilized distilled water after every previous step. This technique is considered as a novel effective procedure. Indirect micropropagation on MS medium supplemented with 2 mg·L-1 2,4-D succeeded in narcissus callus induction which regenerated into shoots on MS medium supplemented with 1 mg.L-1 IBA + 1mg.L-1 TIBA + 1 gm.L-1 of activated charcoal. The same medium with activated charcoal sowed high yield in a direct micropropagation protocol. Average sho...