Studies on Optimization of Papain Hydrolysis Conditions for Release of Glycosaminoglycans from the Chicken Keel Cartilage | Chapter 12 | New Visions in Science and Technology Vol. 1
The goal of this study was to find the best papain hydrolysis conditions for getting glycosaminoglycans from chicken keel cartilages (pH, E/S ratio, incubation temperature, and hydrolysis time). Glycosaminoglycans (GAGs) are natural biocompounds found in sharks, pigs, cows, chickens, and other animals that combine to generate cartilage tissuses. They can be extracted from shark, pig, cow, chicken, and other animal cartilage. GAGs contain chondroitin sulphate (CS), a functional food supplement that is used to prevent and treat arthritis and eye disorders. As a result, GAGs were extracted from byproducts of the cattle and poultry slaughter industries in order to determine the CS cont. Response surface approach was used to find the best hydrolysis conditions in this investigation (RSM). pH (x1), enzyme concentration (x2), incubation temperature (x3), and hydrolysis duration were the independent variables (x4). The variables had a substantial impact on the content of GAGs, according to the...