New Fibrinogenases Isolated From Marine Hydrobiont Adamussium colbecki | Chapter 13 | New Insights on Chemical Research Vol. 1
Aims: Enzymes that affect hemostasis have been isolated from different sources. Fibrinogenolytic enzymes because of their role in dissolving of blood clots as well as preventionof their formation have attracted special medical and scientific attention. The main goal of current research was to analyze marine hydrobionts (an example of the Antarctic scallop Adamussium colbecki) of the Antarctic region on the presence of potential fibrinogenolytic enzymes. Methodology: The fraction that consists of fibrinogenases was obtained by affinity chromatography on Blue Sepharose column. SDS-PAGE was applied for the determination of protein composition in the obtained fraction. The proteolytic activity was monitored by the zymographic technique. Fibrinogenolytic activity and activity toward collagen were assessed by incubation of samples with fibrinogen or collagen, respectively followed by SDS-PAGE analysis. To test for substrate specificity, the fraction of fibrinogenases was incubated wit...