Morphological Characterization and Tissue Culture of Medicinal Plant Aloe vera L. | Chapter 14 | Advances and Trends in Biotechnology and Genetics Vol. 3
The morphological characterization and micro propagation were carried out on Aloe vera (Aloe barbadensis Miller) accessions collected from different locations in India were discussed in this book chapter. The experiments were conducted on 12 Aloe accessions and the leaf length, width, thickness, stem length and thickness were measured using verniercalipers. The plantlets were chemically treated with 2.5% (w/v) of sodium hypochlorite which is an alternative safer surface sterilization method for replacement of mercury chloride by using shoot tip as explants. The sterilization results indicate that the highest number (93%) of viable explants (10) with the best regeneration potential was in Murashige and Skoog (MS) medium compared with other treatments. Multiple shoots/explants (35) were found in MS medium containing 6-benzylaminopurine (BAP, 1.5 mgL -1 ; kinetin (0.5 mgL -1 ), indole-3-butyric acid (IBA, 0.2 mg L- 1 ), indole-3-acetic acid (IAA, 0.1 mg L -1 ), and naphthalene acetic a...