Extraction of High-quality Genomic DNA from Different Plant Orders Applying a Modified CTAB-Based Method | Chapter 07 | Modern Research in Botany Vol. 1
Background: Reliable measurement of DNA concentration and purity is important for almost all molecular genetics studies. Different plant species have varying levels of polysaccharides, polyphenols and other secondary metabolites which combine with nucleic acids during DNA isolation and further affect the quality of the extracted DNA. The current extraction protocol is based-upon the conventional CTAB method with further modifications for the extraction of DNA from variable plant seeds and crops belong to seven different orders The principle modifications currently employed for DNA extraction involved the use of higher CTAB concentration and higher levels of β-Mercaptoethanol. Additionally, higher concentrations of sodium chloride and potassium acetate were added simultaneously with absolute ice cold isopropanol for the precipitation of DNA free from polysaccharides. Results and Conclusion: The prescribed modifications in the present method establish a quick and efficient stan...