Electrochemical Evaluation of Interactions from Cratylia mollis Seed Lectin with Inhibitor Carbohydrate | Chapter 02 | Advances and Trends in Biotechnology and Genetics Vol. 1
Aims: To evaluate the charge transference and the electrochemical potential of Cratylia mollis seed lectin, Cramoll 1,4, adsorbed on Nafion beads after the interaction of glucose ligand. Study Design: Based on detection of electrochemical currents and potentials of Cramoll 1,4 by electrochemical techniques. Place and Duration of Study: Department of Chemistry, Catholic University of Pernambuco, between April 2014 and November 2015. Methodology: Cyclic Voltammetry (CV) was performed in an electrochemical cell containing three electrodes (a calomel electrode, a platinum wire counter electrode and a platinum electrode), connected to a potentiostat to obtain electrochemical currents related to the charge transference. An electrochemical cell containing a calomel electrode and a platinum electrode coupled to a multimeter was used to register the potentials. A saline solution was used as support to control the charge distribution inside of the cell. Cramol...