Electrochemical Evaluation of Interactions from Cratylia mollis Seed Lectin with Inhibitor Carbohydrate | Chapter 02 | Advances and Trends in Biotechnology and Genetics Vol. 1
Aims:
To evaluate the charge transference and the electrochemical potential of
Cratylia mollis seed lectin, Cramoll 1,4, adsorbed on Nafion beads after the
interaction of glucose ligand.
Study
Design: Based on detection of electrochemical
currents and potentials of Cramoll 1,4 by electrochemical techniques.
Place
and Duration of Study: Department of Chemistry, Catholic
University of Pernambuco, between April 2014 and November 2015.
Methodology:
Cyclic Voltammetry (CV) was performed in an electrochemical cell containing
three electrodes (a calomel electrode, a platinum wire counter electrode and a
platinum electrode), connected to a potentiostat to obtain electrochemical
currents related to the charge transference. An electrochemical cell containing
a calomel electrode and a platinum electrode coupled to a multimeter was used
to register the potentials. A saline solution was used as support to control
the charge distribution inside of the cell.
Cramoll 1,4-glucose interaction was evaluated in the concentration of 100, 200
and 300 mM of glucose.
Results:
CV measurements showed significant charge transference after Cramoll
1,4-glucose interaction. Cathodic and anodic peaks paired near 100 mV were
detected in the range 100-300 mM glucose, achieving a maximum current response
of 1300 μA, approximately. Positive electrochemical potentials of Cramoll 1,4
adsorbed to Nafion-beads was achieved showing a linear behaviour with the increase
of glucose concentration at 300 mM.
Conclusion:
The system is useful for characterization of lectin-carbohydrate interactions
and as a glucose sensor to estimate the activity of lectins. The use of cyclic
voltammetry allowed the detection of a redox potential of 100 mV to Cramoll
1,4/Nafion-beads through the interaction with different glucose concentrations
and constitutes another approach to determine Cramoll 1,4 activity. Electrochemical
potential measurements generate a linear response for glucose concentrations,
being an alternative for glucose sensing.
Charge transference of lectins is a great phenomenon to evaluate lectin-carbohydrate
interactions in biological systems.
Author(s) Details
Sandra Rodrigues de Souza
Departamento de Educação,
Universidade Federal Rural de Pernambuco, UFRPE, Rua Dom Manuel de Medeiros,
S/N, Dois Irmãos, Recife-PE, CEP 52171-900, Brasil.
Priscila Marcelino dos
Santos Silva
Departamento de Bioquímica,
Centro de Biociências, Universidade Federal de Pernambuco, UFPE, Avenida
Professor Moraes Rego, S/N, Cidade Universitária, Recife-PE, CEP 50670-420,
Brasil.
Katia Cristina Silva de
Freitas
Departamento de Química,
UFRPE, Rua Dom Manuel de Medeiros, S/N, Dois Irmãos,Recife-PE, CEP 52171-900,
Brasil.
Maria Tereza dos Santos
Correia
Departamento de Bioquímica,
Centro de Biociências, Universidade Federal de Pernambuco, UFPE, Avenida
Professor Moraes Rego, S/N, Cidade Universitária, Recife-PE, CEP 50670-420,
Brasil.
Luana Cassandra Breitenbach
Barroso Coelho
Departamento de Bioquímica,
Centro de Biociências, Universidade Federal de Pernambuco, UFPE, Avenida Professor
Moraes Rego, S/N, Cidade Universitária, Recife-PE, CEP 50670-420, Brasil.
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