B P International

Background: Mast cells (MC) secrete a variety of molecules that are implicated in either promoting tumour growth or may act in an opposite manner in other tumour types.  Aim: To evaluate the association of mast cells in the different grades of Oral Squamous Cell Carcinoma (OSCC).  Material and Methods: 45 diagnosed cases each of well, moderate and poorly differentiated OSCC were identified from the institutional archival material. Slides were made of 5 µm thickness and stained with Hematoxylin and eosin and 1% Toluidine blue stain. Each section was evaluated for mast cells in peritumoural, intratumoural locations and at the invasive front of the tumour. The results obtained were subjected to statistical evaluation by an SPSS version 19 using Chi square test, Anova and Post hoc Tuckey tests.  Results: An inverse relation was observed between MC count with grade of the tumour with maximum MC located in the peritumoural area followed by the invasive front and the least in t...

Aims: Melatonin (MLT) exerts oncostatic effects on numerous tumour types presumably by inhibiting cell proliferation and promoting apoptosis. The primary aim of this study was to investigate melatonin-induced changes in gene expression patterns in two different oral squamous carcinoma cell lines (OSCC). Methodology: This was a prospective non-randomized experimental study design that was conducted at Department of Biomedical Sciences at the University of Nevada, Las Vegas – School of Dental Medicine between May 2016 and March 2017. The SCC25 and CAL27 cell lines were cultured with and without MLT (10 ug/mL) for 72 hours, and total cellular RNA was isolated and converted to cDNA. The expression of 92 genes associated with the molecular mechanisms of cancer and four endogenous control genes were examined by qRT-PCR. The fold change with respect to control levels were calculated using the comparative method or delta-delta ddCt algorithm. Results: Gene expression was compared betw...