B P International

Olea europaea is an economically important tree species native to Mediterranean region. The present study is focused on developing the regeneration protocol and production of true-to-type plants of Olea europaea L. cv Barnea with commercial importance. Olive tree is a long lived evergreen tree but has a long juvenile phase and reaches its full production stage at 25 - 30 years of age. Traditionally, for many years, they have been propagated through cutting, grafting, vegetative propagation and in recent years, through air layering through plant tissue culture methods this problem of long juvenility can be overcome in addition large number of pathogen free plants can be produced. Nodes from adventitious shoots were surface sterilized and inoculated in different tissue culture media such as Woody Plant Media (WPM), Murashige and Skoog media (MS), Rugini Olive media (OM), Schenk and Hildebrandt media (SH) and Multiplication media (Combination of half strength MS and half strength Rugini...

The species Syagrus oleracea is a native palm tree, present in highly impacted environments of the Brazilian semiarid. This species is exploited for use in the manufacture of folk crafts and for human and animal feeding. The objective of this study was to characterize the diversity and genetic structure of individuals from different populations, located in anthropic regions of the Brazilian semiarid region, in the state of Minas Gerais. This information is important for species management and conservation strategies. Thus, we used ISSR molecular markers in 157 individuals in seven populations of S. oleracea. Looking at the results, one can observe that amplifications of 109 locos occurred and that an average of 18, 17 alleles were found on each primer, values that demonstrated that the ISSR technique used was highly efficient. The genetic diversity indexes were observed, showing expected heterozygosity (He) and Shannon index (I) of 0.260 and 0.383, respectively. The number of allele...