Rapid Multiplication for Producing Quality Planting Material of Olive [Olea europaea L. cv. Barnea] through Plant Tissue Culture and It’s Commercial Application | Chapter 03 | Modern Research in Botany Vol. 1
Olea europaea is an economically
important tree species native to Mediterranean region. The present study is
focused on developing the regeneration protocol and production of true-to-type
plants of Olea europaea L. cv Barnea with commercial importance. Olive tree is a
long lived evergreen tree but has a long juvenile phase and reaches its full
production stage at 25 - 30 years of age. Traditionally, for many years, they
have been propagated through cutting, grafting, vegetative propagation and in
recent years, through air layering through plant tissue culture methods this
problem of long juvenility can be overcome in addition large number of pathogen
free plants can be produced. Nodes from adventitious shoots were surface
sterilized and inoculated in different tissue culture media such as Woody Plant
Media (WPM), Murashige and Skoog media (MS), Rugini Olive media (OM), Schenk
and Hildebrandt media (SH) and Multiplication media (Combination of half
strength MS and half strength Rugini OM) in which culture establishment was
best obtained in multiplication media supplemented with 1 mg/l zeatin.
Different treatment was given to induce shoot proliferation and the highest
shoot number 4.2±0.4 was obtained in multiplication media enriched with 2.0
mg/l zeatin and silver nitrate at 2.0 mg/l. The in-vitro derived shoots were
assessed for genetic fidelity using different molecular markers Random
Amplification of Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat
(ISSR). Monomorphic bands were obtained between the mother DNA and in-vitro
derived progenies which indicated that there is genetic stability in the
in-vitro regenerated plants. Commercial analysis was done with respect to
Industrial Use. The global market for olive is estimated to grow at a compound
annual growth rate of 4.5% in the forecast period of 2019-2024. In the
production of olive, Spain holds the largest share of world production and
Italy holds the second largest share. Although in recent years, the world has
begun to see olive from various new regions. The present study is the
initiative step towards propagation of Olive cultivar in South Asian region.
Author(s) Details
Dr. (Mrs.) Susmita Shukla
Applied Plant Biotechnology
Research Laboratory, Centre for Plant and Environmental Biotech, Amity
Institute of Biotechnology, Amity University, Noida (U.P), India.
Dr. Shiv Kant Shukla
Biotech Consortium India
Limited (BCIL), Anuvrat Bhawan, 210 DDU Marg, New Delhi 02, India.
Taramla Raman
Applied Plant Biotechnology
Research Laboratory, Centre for Plant and Environmental Biotech, Amity
Institute of Biotechnology, Amity University, Noida (U.P), India.
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