The Antimicrobial Effects of Secondary Metabolites of Anguillan Fungi | Chapter 09 | Theory and Applications of Microbiology and Biotechnology Vol. 1
Introduction: Many drugs have been isolated from fungal species. This study aims at identifying fungal species isolated in Anguilla to determine the antimicrobial effect of their secondary metabolites from pure culture against Staphylococcus aureus by demonstrating the presence of a zone of inhibition in the culture plate. Methods: Samples were cultured and sub-cultured to isolate pure culture on potato dextrose yeast agar (PDYA) and chosen for further studies by the presence of surface exudates. Those cultures that produced copious amounts of surface exudates were examined for antimicrobial effects by further testing. Results: Antimicrobial testing of MB0725C (P. digitatum) samples did not result in any evidence of antimicrobial properties. However, MB0725A (P. chrysogenum) punch biopsy-agar overlay produced an 11 mm ZOI, whereas crude exudates testing resulted in a 27 mm ZOI. Crude culture filtrate of potato dextrose broth (PDB) did not result in any ZOI for either MB072...