Skip to main content

The Antimicrobial Effects of Secondary Metabolites of Anguillan Fungi | Chapter 09 | Theory and Applications of Microbiology and Biotechnology Vol. 1

Introduction: Many drugs have been isolated from fungal species. This study aims at identifying fungal species isolated in Anguilla to determine the antimicrobial effect of their secondary metabolites from pure culture against Staphylococcus aureus by demonstrating the presence of a zone of inhibition in the culture plate.

Methods: Samples were cultured and sub-cultured to isolate pure culture on potato dextrose yeast agar (PDYA) and chosen for further studies by the presence of surface exudates. Those cultures that produced copious amounts of surface exudates were examined for antimicrobial effects by further testing.

Results: Antimicrobial testing of MB0725C (P. digitatum) samples did not result in any evidence of antimicrobial properties. However, MB0725A (P. chrysogenum) punch biopsy-agar overlay produced an 11 mm ZOI, whereas crude exudates testing resulted in a 27 mm ZOI. Crude culture filtrate of potato dextrose broth (PDB) did not result in any ZOI for either MB0725A or C. Sensitivity testing on samples collected from Yeast Extract Lactose Broth (YELB) on Day 3, Day 6, Day 9 and Day 12 resulted in ZOI of 11 mm, 13, 15 and 17 mm respectively. The change in pH for MB0725A liquid culture in PDB versus YELB was significantly different (N=12, P<0.0001).

Conclusion: MB0725A was an excellent producer of surface exudates and further experiments showed that its secondary metabolites had antimicrobial effects against Staphylococcus aureus using Kirby-Bauer sensitivity testing.

Author(s) Details

Michael Bennardo
Department of Medical Microbiology and Immunology, Saint James School of Medicine, BWI, Anguilla.

Adekunle Sanyaolu
Federal Ministry of Health, Abuja, Nigeria.

Subhajit Dasgupta
Department of Medical Microbiology and Immunology, Saint James School of Medicine, BWI, Anguilla.

View Books: http://bp.bookpi.org/index.php/bpi/catalog/book/95

Labels

Labels:

Comments

Post a Comment

Popular posts from this blog

Greening Regional Airports: A Vision for Carbon Neutral Infrastructure | Chapter 12 | Contemporary Perspective on Science, Technology and Research Vol. 3

 This study provides an overview of the energy demand of a regional airport, divided into individual time horizons. The electrification of aircraft systems raises the question of whether airports will be among the largest electricity consumers in our infrastructure in the future. Sustainability and especially emission reductions are significant challenges for airports that are currently being addressed. The Clean Sky 2 project GENESIS addresses the environmental sustainability of hybrid-electric 50-passenger aircraft systems in a life cycle perspective to support the development of a technology roadmap for the transition to sustainable and competitive electric aircraft systems. This article originates from the GENESIS research and describes various options for ground power supply at a regional airport. Potential solutions for airport infrastructure with a short (2030), medium (2040), and long (2050) time horizon are proposed. In addition to the environmental and conservation benefi...
Post a Comment
Read more

Occipital Dermal Sinus Tract Causing Craniospinal Infection: A Review | Chapter 13 | New Visions in Medicine and Medical Science Vol. 4

  Dermal sinus is a rare congenital condition characterized by a pathological tract connecting the skin to deeper tissues of the central nervous system, potentially leading to severe infectious complications. It arises from a failure in the separation of ectodermal layers during early gestation. Diagnosing dermal sinus tract in newborns requires a careful physical examination, focusing on midline dimples in the occipital region associated with cutaneous abnormalities like hairy nevus or hyperpigmentation. The presence of drainage, abnormal hair distribution, or localized swelling may indicate a sinus tract. Regular examination for dimples or sinuses is recommended for infants and children with recurrent meningitis or infections. Early detection is crucial to prevent severe complications like meningitis, with Staphylococcus aureus being a common causative organism. Neuroradiological studies, including computed tomography (CT) scan and magnetic resonance imaging (MRI), with histopath...
Post a Comment
Read more

Alkali Element Modification of Glucose Molecules as a Method to Dissolve Cancer Cells | Chapter 12 | New Visions in Medicine and Medical Science Vol. 4

  The present study highlights about alkali element modification of glucose molecules as a method to dissolve cancer cells. The central regulation of the mechanisms governing cell proliferation has little effect on cancer cells. Cancer cells are entirely independent of the central command and divide and proliferate on their own, making it challenging to activate their response mechanism. Precisely, this is the reason why they are at risk to the health of humans and/or any biological entities. Instead of trying to reconnect the central command of the growth control mechanism to cancer cells that are already out of the range, we present a method of using the cancer cell’s own irresponsive and uncontrolled growth mechanism to their disadvantage and destroy the cancer cells. We found that this is achievable in an atomic/molecular level study of the glucose molecule, which is the primary food source used for growth and energy generation by all cells in the body, including the cancer cel...
Post a Comment
Read more