Rapid and Mass Propagation of Hybanthus enneaspermus (L.) F. Muell. from Shoot Tip and Nodal Explants | Chapter 11 | Modern Research in Botany Vol. 1
A rapid and efficient protocol for in vitro propagation of Hybanthus enneaspermus (L.) F. Muell. (Violaceae) has been developed from the shoot tip and nodal explants. The explants were cultured on MS basal medium supplemented with different concentrations of cytokinins, viz., BAP and Kin, ranging from 5 µM to 25 µM, either individually or in combinations of both these cytokinins for shoot induction. Shoot buds of both the explants proliferated on MS medium supplemented with both cytokinins. The best response was observed on MS medium containing 15 µM BAP. Subsequently the optimum concentration of BAP (15 µM) was combined with different concentrations of Kin ranging from 2 µM to 10 µM. Maximum number of 28.6 ± 0.90 and 36.8 ± 1.54 shoots were produced on MS medium containing 15 µM BAP + 6 µM Kin from the shoot tip and nodal explants respectively. The regenerated shoots were transferred to rooting medium containing auxins at different concentrations ranging from 2 µM to 10 µM of ...