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Phenotypic and Genotypic Characterization of Thermophilic Campylobacter Isolated from Chicken in Côte d’Ivoire | Chapter 9 | Theory and Applications of Microbiology and Biotechnology Vol. 3

Thermophilic Campylobacters are major causes of gastroenteritis in human. The main risk factor of infection is consumption of contaminated or by cross-contaminated poultry meat. In Coteˆ d’Ivoire, gastroenteritis is usually observed but no case of human campylobacteriosis has been formally reported to date. The aims of this study were to determine prevalence and antimicrobial resistance of Campylobacter jejuni and Campylobacter coli isolated from chickens ceaca in commercial slaughter in Abidjan. Between May and November 2009, one hundred and nineteen (119) chicken caeca samples were collected and analyzed by passive filtration method followed by molecular identification (PCR). From these 119 samples, 76 (63.8%) were positive to Campylobacter tests. Among the positive colonies, 51.3% were C. jejuni and 48.7% were C. coli. Of the 39 C. jejuni isolates, 79.5%, 38.5%, 17.9%, 10.3%, and 7.7% were, respectively, resistant, to nalidixic acid, ciprofloxacin, amoxicillin, erythromycin, and ...

Antibiotics Susceptibility Profile of Bacterial Isolates from Urinary Tract Infection among Honeymoon Women in Iraq | Chapter 13 | Advances and Trends in Biotechnology and Genetics Vol. 3

Urinary Tract Infections (UTIs) is an infection caused by the presence and growth of microorganism anywhere in the urinary tract. This study aims at determining the most common microorganism that causes UTI in honeymoon women, detection the most effective antimicrobial agents that causative agents of UTI and select the best antibiotics for treated UTI. One hundred and thirty specimens were collected from women suspected with UTI {honeymoon (95) and other women (35)} in al-Qassim hospital; during the period (January 2012 to 30th September 2012). The bacterial isolates were identified tested for antibiotics sensitivity test6. Honeymoon women were showing higher rate of UTI than other women in (87 from 95) (91.5%) and (19 from 35) (54.3%) respectively. E. coli (46%) and Staphylococcus aureus (42%) were predominant isolate in honeymoon. Regarding Antibiotics sensitivity test, E. coli and Staphylococcus aureus showed highly sensitive to Nitrofurantoin (100%), (94.5%) followed by Amikacin ...

Biochemical Characterization of Xanthomonas axonopodis pv. malvacearum Isolated from Infected Cotton Plant and It’s in vitro Sensitivity against Some Selected Chemicals | Chapter 10 | Current Research in Science and Technology Vol. 3

Aim: The aim of our study is to isolate, identify and pathogenicity test of Xanthomonas axonopodis pv. malvacearum (Xam) causing bacterial blight of cotton and to determine the comparative efficacy of some selected chemicals and antibiotic in controlling Xam in vitro. Place and Duration of the Study: This study was carried out at the Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh from January, 2012 to December, 2013. Methodology: Xanthomonas axonopodis pv. malvacearum was isolated from infected leaves, stems, branches, cotyledons and bolls of cotton. Biochemical characterization and in vitro sensitivity were evaluated as per the standard methods as described in materials and methods. Results: The bacterium was gram negative, rod shaped and showed positive results in KOH solubility, starch hydrolysis, catalase, citrate utilization, motility indole ureas agar (MIU), gelatin liquefaction test and oxidase test. It produced, slightly r...

Use of Clinical and Laboratory Clue to Diagnose Anaerobic Infections in Limited Resource Setting | Chapter 08 | Theory and Applications of Microbiology and Biotechnology Vol. 2

Increase in life threatening infection despite the use of antimicrobial among patient’s in limited resource areas has made it imperative to investigate bacteria causing infections. However, comprehensive anaerobic bacteriology of clinical specimens is expensive and time consuming procedure. With advance in diagnostic techniques, the role of anaerobic bacteria in the etiology of various infection has become increasingly recognized. While a number of infections or intoxication due to anaerobic bacteria or their toxins arise from exogenous source such as the soil the majority are often endogenous origin arising frequently from the intestinal tract, oral cavity or the female genital tract. This chapter therefore highlight pointers for clinical and laboratory clue of anaerobic infection, additionally practical laboratory procedure to Isolate and identify anaerobes have been explained. Author(s) Details Sima E. Rugarabamu Department of Microbiology and Immunology, Muhimbili Univer...

The Antibiotic Resisting Profile of Salmonella spp Isolated from the Sewage of the Campus of the University of Cocody, Abidjan, Côte d’Ivoire | Chapter 04 | Current Trends in Disease and Health Vol. 1

Background: Recent studies have shown that wastewater is contaminated by salmonella sp., pathogenic antibiotics-resisting bacteria. Using wastewater in periurban agriculture in Abidjan is likely to be the source of food-borne diseases such as salmonellosis. However, what we know about these resistant salmonella spp. in wastewater is limited in the country. Aims: This study aims to establish the susceptibility profile of Salmonella spp., isolated from wastewater to antibiotics and to antimetabolite commonly used by medical practitioners. Study Design: Spatio-temporal variation was taken into account. Place and Duration of Study: The study took place from August 2008 to January 2009 at the main campus of the University of Cocody in Abidjan. Methodology: Sampling was done on a weekly basis. Wastewater samples were collected at four different sewers in the campus area. Salmonella sp was isolated by a standard method of laboratory. The resistance of...

Brucellosis of Testis and Epididymis: An Update of the Literature | Chapter 09 | Current Trends in Medicine and Medical Research Vol. 4

Background: Brucellosis is a zoonotic disease which has the ability to afflict a number of organs and tissues.   Brucellosis   epididymoorchitis   (BEO),   a   complication   of   human   brucellosis,   can   emanate   in complications. In brucellosis non-endemic areas, some clinicians may be unfamiliar with the disease entity which may lead to delay in the diagnosis. Aim: To review the literature on BEO, in order to document its presentation, diagnosis, management and outcome following treatment as well as update the literature related to the disease. Methods: Various internet data bases were usedto obtain literature on BEO. Results   /   Literature   Review: BEO (epididymitis   plus   or   minus   orchitis)   is   a   complication   of   an infection   caused   by   brucella   species   which   can   be ...