Phenotypic and Genotypic Characterization of Thermophilic Campylobacter Isolated from Chicken in Côte d’Ivoire | Chapter 9 | Theory and Applications of Microbiology and Biotechnology Vol. 3
Thermophilic Campylobacters are major causes of
gastroenteritis in human. The main risk factor of infection is consumption of
contaminated or by cross-contaminated poultry meat. In Coteˆ d’Ivoire,
gastroenteritis is usually observed but no case of human campylobacteriosis has
been formally reported to date. The aims of this study were to determine
prevalence and antimicrobial resistance of Campylobacter jejuni and
Campylobacter coli isolated from chickens ceaca in commercial slaughter in
Abidjan. Between May and November 2009, one hundred and nineteen (119) chicken
caeca samples were collected and analyzed by passive filtration method followed
by molecular identification (PCR). From these 119 samples, 76 (63.8%) were
positive to Campylobacter tests. Among the positive colonies, 51.3% were C.
jejuni and 48.7% were C. coli. Of the 39 C. jejuni isolates, 79.5%, 38.5%,
17.9%, 10.3%, and 7.7% were, respectively, resistant, to nalidixic acid,
ciprofloxacin, amoxicillin, erythromycin, and gentamicin. Among the 37 isolates
of C. coli, 78.4%, 43.2%, 13.5%, 8.1%, and 0% were resistant, respectively, to
the same antibiotics. On the other hand, detection of virulence putative gene
shows presence of cadF in 100 % of tested strains. In addition, cdtA, cdtB and
cdtC genes were detected in 100%; 69.43% and 71.06% respectively of C. jejuni
isolates. Moreover, only cdtA gene of cdt genes was detected in 12.82% of C.
coli strains tested in this study. In conclusion, we reported in this study the
presence of high Campylobacter contamination of the studied chickens. Molecular
identification of the bacteria was performed and determination of high
resistance to antimicrobials of the fluoroquinolone family was revealed.
Author(s) Details
Author(s) Details
Dr. Goualié Gblossi
Bernadette
Laboratoire de Biotechnologies, Agriculture et Valorisation des Ressources Biologiques, Unité de Formation et de Recherche en Biosciences, Université de Cocody-Abidjan, 01 BP 582, Abidjan, Cote d’Ivoire.
Dr. Akpa Eric Essoh
Laboratoire de Biotechnologies, Agriculture et Valorisation des Ressources Biologiques, Unité de Formation et de Recherche en Biosciences, Université de Cocody-Abidjan, 01 BP 582, Abidjan, Cote d’Ivoire.
Dr. Kakou-N’Gazoa Elise Solange
Institut Pasteur de Côte d’Ivoire, 01 BP 490, Abidjan, Cote d’Ivoire.
Dr. Guessennd Natalie
Institut Pasteur de Côte d’Ivoire, 01 BP 490, Abidjan, Cote d’Ivoire
Dr. Bakayoko Souleymane
Institut Pasteur de Côte d’Ivoire, 01 BP 490, Abidjan, Cote d’Ivoire.
Laboratoire de Biotechnologies, Agriculture et Valorisation des Ressources Biologiques, Unité de Formation et de Recherche en Biosciences, Université de Cocody-Abidjan, 01 BP 582, Abidjan, Cote d’Ivoire.
Dr. Akpa Eric Essoh
Laboratoire de Biotechnologies, Agriculture et Valorisation des Ressources Biologiques, Unité de Formation et de Recherche en Biosciences, Université de Cocody-Abidjan, 01 BP 582, Abidjan, Cote d’Ivoire.
Dr. Kakou-N’Gazoa Elise Solange
Institut Pasteur de Côte d’Ivoire, 01 BP 490, Abidjan, Cote d’Ivoire.
Dr. Guessennd Natalie
Institut Pasteur de Côte d’Ivoire, 01 BP 490, Abidjan, Cote d’Ivoire
Dr. Bakayoko Souleymane
Institut Pasteur de Côte d’Ivoire, 01 BP 490, Abidjan, Cote d’Ivoire.
Prof. Niamké Lamine Sébastien
Laboratoire de Biotechnologies, Agriculture et Valorisation des Ressources Biologiques, Unité de Formation et de Recherche en Biosciences, Université de Cocody-Abidjan, 01 BP 582, Abidjan, Cote d’Ivoire.
Prof. Dosso Mireille
Institut Pasteur de Côte d’Ivoire, 01 BP 490, Abidjan, Cote d’Ivoire.
View Book: - http://bp.bookpi.org/index.php/bpi/catalog/book/154
Laboratoire de Biotechnologies, Agriculture et Valorisation des Ressources Biologiques, Unité de Formation et de Recherche en Biosciences, Université de Cocody-Abidjan, 01 BP 582, Abidjan, Cote d’Ivoire.
Prof. Dosso Mireille
Institut Pasteur de Côte d’Ivoire, 01 BP 490, Abidjan, Cote d’Ivoire.
View Book: - http://bp.bookpi.org/index.php/bpi/catalog/book/154
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