Effects of Lead on Different Seedling Growth Attributes of Cowpea (Vigna unguiculata L.)| Chapter 04 | New Perspectives in Agriculture and Crop Science Vol. 1
Aims: This study was conducted to determine the toxicity and tolerance to lead by the seedling growth of an important country legume crop cowpea (Vigna unguiculata L). Study Design: The seedlings of cowpea were grown in sand culture at 0, 20, 40, 60, 80 and 100 ppm of metal ions of lead salt as lead acetate. Place and Duration of Study: The experimental site is located in the Department of Botany at the Karachi University Campus, Pakistan in 2011. Methodology: The healthy seeds of Vigna unguiculata L. were surface sterilized with 0.2% solution of sodium hypochlorite (NaOCL) for one minute to avoid any fungal contamination. The sand was collected from the construction site of the Karachi University washed 2-3 times with tap water, distilled water and with 5% HCl to remove any types of impurities from the soil. Seedlings were grown in sand culture at 0, 20, 40, 60, 80 and 100 ppm of metal ions of lead salt as lead acetate. The Hoagland solution was used for the s...