Cluster of Differentiation 4, Chemokine Receptor 5 and C-X-C Chemokine Receptor Type 4 in Different Cell Lines After Infection with HIV: Expression Analysis of Cell Surface Markers | Chapter 16 | New Frontiers in Medicine and Medical Research Vol. 14
The human immunodeficiency virus (HIV) infection of the host cell begins with the virus's entry into the cell. A retrovirus, the human immunodeficiency virus (HIV), causes acquired immune deficiency syndrome (AIDS) (AIDS). Cluster of differentiation 4 (CD4) + T lymphocytes, which express co-receptors, chemokine receptor 5 (CCR5) or C-X-C chemokine receptor type 4, are HIV's main targets (CXCR4). The HIV-1 envelope glycoprotein 120 (Gp120) interacts to its major receptor CD4, which is a member of the immunoglobulin superfamily that promotes TCR-mediated signalling and is necessary for infection. Recent research on the levels of cell surface marker expression by infecting CD4+ T cells We used quantitative real time PCR (qRT-PCR) to examine the expression of cell surface markers gp120, CD4, CCR5, and CXCR4 levels in Jurkat, SupT1 cells, and PBMCs at different time intervals after infection. Our findings demonstrate a 5-fold rise in gp120 expression 1 hour after infection and a 1.5...