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Cluster of Differentiation 4, Chemokine Receptor 5 and C-X-C Chemokine Receptor Type 4 in Different Cell Lines After Infection with HIV: Expression Analysis of Cell Surface Markers | Chapter 16 | New Frontiers in Medicine and Medical Research Vol. 14

The human immunodeficiency virus (HIV) infection of the host cell begins with the virus's entry into the cell. A retrovirus, the human immunodeficiency virus (HIV), causes acquired immune deficiency syndrome (AIDS) (AIDS). Cluster of differentiation 4 (CD4) + T lymphocytes, which express co-receptors, chemokine receptor 5 (CCR5) or C-X-C chemokine receptor type 4, are HIV's main targets (CXCR4). The HIV-1 envelope glycoprotein 120 (Gp120) interacts to its major receptor CD4, which is a member of the immunoglobulin superfamily that promotes TCR-mediated signalling and is necessary for infection. Recent research on the levels of cell surface marker expression by infecting CD4+ T cells We used quantitative real time PCR (qRT-PCR) to examine the expression of cell surface markers gp120, CD4, CCR5, and CXCR4 levels in Jurkat, SupT1 cells, and PBMCs at different time intervals after infection. Our findings demonstrate a 5-fold rise in gp120 expression 1 hour after infection and a 1.5-fold increase in relative CD4 and CCR5 expression 2 hours after infection, although CXCR4 showed differential up-regulation in various cell lines, which requires additional investigation.


Author (S) Details

S. L. Balakrishna
Department of Biotechnology and Bioinformatics, School of Life Sciences-Centre for Advanced Studies, University of Hyderabad, P.O. Central University, Hyderabad - 500 046, Telangana, India.

Bharti Gupta
Department of Biotechnology, Faculty of Science, Indira Gandhi National Tribal University, Amarkantak, 484887, Madhya Pradesh, India.

Parikipandla Sridevi
Department of Biotechnology, Faculty of Science, Indira Gandhi National Tribal University, Amarkantak, 484887, Madhya Pradesh, India.


View Book :- https://stm.bookpi.org/NFMMR-V14/article/view/3398

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