Activity Guided Fractionation with Antimicrobial Evaluation of Pergularia tomentosa L. (Asclepiadacea) Whole Plant | Chapter 03 | Theory and Applications of Microbiology and Biotechnology Vol. 1
This research work aimed to present the activity guided fractionation with antimicrobial evaluation from both crude extract and various fractions obtained from Pergularia tomentosa L. whole plant. The P. tomentosa L. whole plant was extracted with 95% aqueous ethanol; fractionated into acidic, basic, polar and nonpolar fractions. All fractions with the crude extract were screened for both antimicrobial and minimum inhibitory concentration (MIC) potentialities. For crude extract, all concentrations (1.5, 0.75, 0.35, & 0.168 mg/ml) indicated marginal antibacterial activity with range of 17, 20, 14 mm zone of inhibition for S. aureus, E. coli and C. albicans. While, both basic fraction showed highest activity against E. coli and C. albicans at 15 mm & 15 mm; along with acidic and methanolic fraction haven large spectrum against S. aureus, E. coli and C. albicans at 13, 12, 12 mm. Moreover hexane did not showed antimicrobial activity for both S. aureus and C. albicans except for ...