Alterations in Oral Cancer Gene Expression in Response to Melatonin | Chapter 12 | New Insights into Disease and Pathogen Research Vol. 3
Aims: Melatonin (MLT) exerts oncostatic effects on numerous tumour types presumably by inhibiting cell proliferation and promoting apoptosis. The primary aim of this study was to investigate melatonin-induced changes in gene expression patterns in two different oral squamous carcinoma cell lines (OSCC). Methodology: This was a prospective non-randomized experimental study design that was conducted at Department of Biomedical Sciences at the University of Nevada, Las Vegas – School of Dental Medicine between May 2016 and March 2017. The SCC25 and CAL27 cell lines were cultured with and without MLT (10 ug/mL) for 72 hours, and total cellular RNA was isolated and converted to cDNA. The expression of 92 genes associated with the molecular mechanisms of cancer and four endogenous control genes were examined by qRT-PCR. The fold change with respect to control levels were calculated using the comparative method or delta-delta ddCt algorithm. Results: Gene expression was compared betw...