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Application of RAPD-PCR for DNA-Fingerprinting of Egyptian Tilapia| Chapter 6 | New Visions in Science and Technology Vol. 1

The DNA fingerprint and similarity of three Egyptian tilapia fish, Oreochromis niloticus, Sarotherodon galilaeus, and Tilapia zilli, were examined using RAPD-PCR. This study used RAPD-PCR (random amplified polymerase chain reaction) with 10 arbitrary primers to evaluate common patterns of genetic variations or similarities among three species of tillapine through DNA fingerprinting analysis. Out of the 10 primers used, 7 primers gave strong sharp distinct bands. The 7 primers yielded a total of 15, 13, 16, 14, 18, 14, and 17 bands. The similarity coefficient of the primers used determines how similar they are. Oreochromis niloticus and Oreochromis galilaius had a 95% resemblance, Oreochromis niloticus and Tilapia zilli had an 80% similarity, and Oreochromis galilaius and Tilapia zilli had a 75% similarity. The genetic distances found were used to create a distance matrix, which was then used to create a dendrogram that linked the analysed species. The results of DNA fingerprinting on t...

Analysis of Genetic Parameters in Chickpea | Chapter 04 | Current Research in Science and Technology Vol. 2

Diverse genotype is the key for any plant selection or hybridization programme; to develop a noble type so as to increase its utility to human. It is not only improves the genetics background of the cultivar but also improve farmers income by reducing losses due to insect-pest and disease attack etc. In view of regards, a research was conducted to analyze the variability and heritability genetic parameter of sixty diverse genotypes of chickpea (Cicer arietinum L.) for 10 different characters. The crop sown in Randomized Block Design (RBD) with two replications during Rabi Season 2014-15 at Pulses Improvement Project, Mahatma Phule Krishi Vidhyapeeth, Rahuri, Maharashtra, India. The evaluation shows that highest range of variability was present for number of pods per plant followed by days to 50 per cent flowering, plant height, 100 seed weight, days to maturity. The highest estimates of heritability along with genetic advance as per cent of mean were recorded for number of pods per...