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Application of RAPD-PCR for DNA-Fingerprinting of Egyptian Tilapia| Chapter 6 | New Visions in Science and Technology Vol. 1

The DNA fingerprint and similarity of three Egyptian tilapia fish, Oreochromis niloticus, Sarotherodon galilaeus, and Tilapia zilli, were examined using RAPD-PCR. This study used RAPD-PCR (random amplified polymerase chain reaction) with 10 arbitrary primers to evaluate common patterns of genetic variations or similarities among three species of tillapine through DNA fingerprinting analysis. Out of the 10 primers used, 7 primers gave strong sharp distinct bands. The 7 primers yielded a total of 15, 13, 16, 14, 18, 14, and 17 bands. The similarity coefficient of the primers used determines how similar they are. Oreochromis niloticus and Oreochromis galilaius had a 95% resemblance, Oreochromis niloticus and Tilapia zilli had an 80% similarity, and Oreochromis galilaius and Tilapia zilli had a 75% similarity. The genetic distances found were used to create a distance matrix, which was then used to create a dendrogram that linked the analysed species. The results of DNA fingerprinting on the investigated fishes can be used to improve genetic features by combining patterns of similarity and the likelihood of hybridization between closely related species. It can also be argued that RAPD-PCR could be a valuable method for measuring genetic variability and the degree of simian mutation.

 

Author (S) Details

H. Soufy
Department of Paracitology, National Research Centre, Egypt.

A. M. Laila
Department of Hydrobiology, National Research Centre, Egypt.

M. K. A. Iman
Department of Hydrobiology, National Research Centre, Egypt.

View Book :- https://stm.bookpi.org/NVST-V1/article/view/3204
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