Phytochemical, GC/MS Analyses and Cytotoxic Effects of Maerua pseudopetalosa (Gilg and Bened.) De Wolf Tuber Fractions | Chapter 09 | Modern Research in Botany Vol. 1
Tuber extracts were subjected to column chromatography technique. Eight fractions were obtained for ethyl acetate and twelve for ethanol. The brine shrimp lethality assay was used for assessment of toxicity. For the first time promising result was shown for ethanolic extract. The fractions F 8 , F 9 , F 11 and F 12 were represented high toxicity equal to 1.25, 7.98, 0.185, 0.041 µg/ml respectively. Also F 7 and F 10 showed toxic effects (89.9, 30.6 µg/ml) whereas F 5 (LC 50 807 µg/ml) was weakly toxic. Ethyl acetate fractions showed moderate toxicity for F 7 and F 4 (299.7 and 375.4 µg/ml), while F 2 , F 6 and F 8 were weakly toxic. However F 1 exhibited high toxic effect. Ethanolic extract which is the highest bioactive extract was subjected to TLC analysis. Tests for secondary metabolites proved the presence of tannins, sterols and alkaloids. Also detection of triterpenes, sterols and flavonoid represented positive results. The fractions F 8 , F 9 , F 11...