Skip to main content

Phytochemical, GC/MS Analyses and Cytotoxic Effects of Maerua pseudopetalosa (Gilg and Bened.) De Wolf Tuber Fractions | Chapter 09 | Modern Research in Botany Vol. 1

Tuber extracts were subjected to column chromatography technique. Eight fractions were obtained for ethyl acetate and twelve for ethanol. The brine shrimp lethality assay was used for assessment of toxicity. For the first time promising result was shown for ethanolic extract. The fractions F8, F9, F11 and F12 were represented high toxicity equal to 1.25, 7.98, 0.185, 0.041 µg/ml respectively. Also F7 and F10 showed toxic effects (89.9, 30.6 µg/ml) whereas F5 (LC50 807 µg/ml) was weakly toxic.

Ethyl acetate fractions showed moderate toxicity for F7 and F4 (299.7 and 375.4 µg/ml), while F2, F6 and F8 were weakly toxic. However F1 exhibited high toxic effect.

Ethanolic extract which is the highest bioactive extract was subjected to TLC analysis. Tests for secondary metabolites proved the presence of tannins, sterols and alkaloids. Also detection of triterpenes, sterols and flavonoid represented positive results.

The fractions F8, F9, F11 and F12 with high cytotoxic values were identified by gas chromatography/ mass spectrometry analysis. Thirty three compounds were detected; which were not recorded in any previous work in the available literature. Fraction 8 and 9 were found to be cytotoxic due to the presence of oleate and linoleate compounds; with more cytotoxicity in fraction 8 as a result of the additional presence of decenoic acid. Also, fraction 12 was more cytotoxic than fraction 11 and this was attributed to the presence of a proline derivative (Proline-N-methyl- butyl ester). This compound might be considered as the cause of the high toxicity of the fraction; since free proline was used as an inhibitor of breast cancer development. Surprisingly, M. pseudopetalosa tubers were used in the folkloric medicine of the natives of the South Blue Nile State in Sudan for the treatment of breast cancer growth without any knowledge of their chemical constituents.

Author(s) Details

Dr. Manal A. Ibrahim
Department of Botany, Faculty of Science and Technology, Omdurman Islamic University, Sudan.

El Bushra E. El Nur
Department of Botany, Faculty of Science, University of Khartoum, Sudan.

View Volume: http://bp.bookpi.org/index.php/bpi/catalog/book/127
Labels:

Comments

Post a Comment

Popular posts from this blog

Greening Regional Airports: A Vision for Carbon Neutral Infrastructure | Chapter 12 | Contemporary Perspective on Science, Technology and Research Vol. 3

 This study provides an overview of the energy demand of a regional airport, divided into individual time horizons. The electrification of aircraft systems raises the question of whether airports will be among the largest electricity consumers in our infrastructure in the future. Sustainability and especially emission reductions are significant challenges for airports that are currently being addressed. The Clean Sky 2 project GENESIS addresses the environmental sustainability of hybrid-electric 50-passenger aircraft systems in a life cycle perspective to support the development of a technology roadmap for the transition to sustainable and competitive electric aircraft systems. This article originates from the GENESIS research and describes various options for ground power supply at a regional airport. Potential solutions for airport infrastructure with a short (2030), medium (2040), and long (2050) time horizon are proposed. In addition to the environmental and conservation benefi...
Post a Comment
Read more

There is No Standard Model of ZFC and ZFC2 | Chapter 03 | Advances in Mathematics and Computer Science Vol. 1

In this Chapter we obtain a contradictions in formal set theories under assumption that these theories have omega-models or nonstandard model with standard part. An possible generalization of Lob’s theorem is considered. Main results are: (i) ¬Con(ZF C+ ∃ M ZFC st ), (ii) ¬Con(N F+ ∃ M NF st ), (iii) ¬Con(ZF C 2 ), (iv) let k be an inaccessible cardinal then ¬Con(ZF C+ ∃ κ), (v) ¬Con(ZF C+ (V=L)), (vi) ¬Con(ZF+ (V=L)). Author   Details: Jaykov Foukzon Israel Institute of Technology, Haifa, Israel Men'kova Elena Romanovna All-Russian Research Institute for Optical and Physical Measurements, Moscow, Russia Read full article: http://bp.bookpi.org/index.php/bpi/catalog/view/46/221/408-1 View Volume: https://doi.org/10.9734/bpi/amacs/v1
Post a Comment
Read more

Study on Processing Method Influences the Effect of Cassava (Manihot esculenta) Consumption on Blood Lipid Profile in Rats | Chapter 8 | Current Research and Development in Chemistry Vol.1

Cassava is consumed in many tropical countries and is fast becoming the major raw material of many diets, especially in the bakery, brewery and pharmaceutical industries. In a study to determine the effects of various formulations of Cassava feeds on blood lipid profile in rats. Forty adult Wistar rats weighing between 120 and 150 g were divided into 4 groups of 10 rats each. The rats were acclimatized for one week; fed with different formulations of Cassava feeds adlibitum, after which the rats were allotted into groups. Group 1 was fed on Garri; group 2 on Cassava flour; group 3 on Tapioca, and the control group (group 4) on normal chow. After 4 weeks, each animal was anaesthetized by exposure to chloroform vapour and blood samples collected by cardiac puncture. Group 1 diet (Garri) significantly increased HDL-cholesterol (HDL-C) concentration and significantly decreased LDL-cholesterol (LDL-C) concentration (p<0.05) compared with the control group. In Group 2 (Cassava flour), t...
Post a Comment
Read more