Activity Guided Fractionation with Antimicrobial Evaluation of Pergularia tomentosa L. (Asclepiadacea) Whole Plant | Chapter 03 | Theory and Applications of Microbiology and Biotechnology Vol. 1
This research work aimed to present the
activity guided fractionation with antimicrobial evaluation from both crude
extract and various fractions obtained from Pergularia tomentosa L. whole
plant. The P. tomentosa L. whole plant was extracted with 95% aqueous ethanol;
fractionated into acidic, basic, polar and nonpolar fractions. All fractions
with the crude extract were screened for both antimicrobial and minimum
inhibitory concentration (MIC) potentialities. For crude extract, all
concentrations (1.5, 0.75, 0.35, & 0.168 mg/ml) indicated marginal
antibacterial activity with range of 17, 20, 14 mm zone of inhibition for S.
aureus, E. coli and C. albicans. While, both basic fraction showed highest
activity against E. coli and C. albicans at 15 mm & 15 mm; along with
acidic and methanolic fraction haven large spectrum against S. aureus, E. coli
and C. albicans at 13, 12, 12 mm. Moreover hexane did not showed antimicrobial
activity for both S. aureus and C. albicans except for E. coli which showed
activity at 12 mm. The study clearly indicated that basic fraction showed
highest antimicrobial activity for selected micro-organisms with lower minimum
inhibitory concentration which ranges from 18.75 µg/ml to 150 µg/ml. Followed
by wider spectrum of antimicrobial activity for acidic and methanolic fraction
against all tested organisms with minimum inhibitory concentration from 75
µg/ml to 150 µg/ml; while 300 µg/ml (MIC) stand for hexane fraction. Thus, P.
tomentosa L. particularly, the basic fraction (alkaloid) and, both acidic and
neutral fractions will be centered areas for further scientific research
findings in isolating an active antimicrobial component therein.
Author(s) Details
Rufai Yakubu
Department of Chemistry,
Federal College of Education Okene, P.M.B. 1026, Kogi State, Nigeria.
Mrs. Fatimah Musa
Department of Chemistry,
Federal College of Education Okene, P.M.B. 1026, Kogi State, Nigeria.
Aminu Lukman
Department of Chemistry,
Federal College of Education Okene, P.M.B. 1026, Kogi State, Nigeria.
Fatima Sheikh
Department of Chemistry,
Federal College of Education Okene, P.M.B. 1026, Kogi State, Nigeria.
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