Rapid and Mass Propagation of Hybanthus enneaspermus (L.) F. Muell. from Shoot Tip and Nodal Explants | Chapter 11 | Modern Research in Botany Vol. 1
A rapid and efficient protocol for in
vitro propagation of Hybanthus enneaspermus (L.) F. Muell. (Violaceae) has been
developed from the shoot tip and nodal explants. The explants were cultured on
MS basal medium supplemented with different concentrations of cytokinins, viz.,
BAP and Kin, ranging from 5 µM to 25 µM, either individually or in combinations
of both these cytokinins for shoot induction. Shoot buds of both the explants
proliferated on MS medium supplemented with both cytokinins. The best response
was observed on MS medium containing 15 µM BAP. Subsequently the optimum
concentration of BAP (15 µM) was combined with different concentrations of Kin
ranging from 2 µM to 10 µM. Maximum number of 28.6 ± 0.90 and 36.8 ± 1.54 shoots were produced on MS
medium containing 15 µM BAP + 6 µM Kin from the shoot tip and nodal explants
respectively. The regenerated shoots were transferred to rooting medium
containing auxins at different concentrations ranging from 2 µM to 10 µM of
IAA, IBA or NAA. The highest number of roots were observed on half strength MS
medium fortified with 4 µM IBA. The plantlets were then hardened and
acclimatized in soil. About 80% of plantlets were survived in the field
condition. A completely randomized design was used in all experiments and
analysis of variance and mean separations were carried out using Duncan’s
Multiple Range Test. Each treatment
factor consists of 10 replicates repeated for 5 times. This protocol would help
ex situ conservation of this medicinal plant.
Author(s) Details
Dr. P. Velayutham
Krishna College of Arts and
Science, Kolluthannipatti, Karur Dt. Tamil Nadu, India.
C. Karthi
ICAR - National Research
Centre for Banana, Tiruchirappali, Tamil Nadu, India.
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