Morphological Characterization and Tissue Culture of Medicinal Plant Aloe vera L. | Chapter 14 | Advances and Trends in Biotechnology and Genetics Vol. 3
The morphological characterization and
micro propagation were carried out on Aloe vera (Aloe barbadensis Miller)
accessions collected from different locations in India were discussed in this
book chapter. The experiments were conducted on 12 Aloe accessions and the leaf
length, width, thickness, stem length and thickness were measured using verniercalipers.
The plantlets were chemically treated with 2.5% (w/v) of sodium hypochlorite
which is an alternative safer surface sterilization method for replacement of
mercury chloride by using shoot tip as explants. The sterilization results
indicate that the highest number (93%) of viable explants (10) with the best
regeneration potential was in Murashige and Skoog (MS) medium compared with
other treatments. Multiple shoots/explants (35) were found in MS medium
containing 6-benzylaminopurine (BAP, 1.5 mgL-1; kinetin (0.5 mgL-1),
indole-3-butyric acid (IBA, 0.2 mg L-1), indole-3-acetic acid (IAA,
0.1 mg L-1), and naphthalene acetic acid (NAA, 0.2 mg L-1).
Based on the shoot proliferation, a maximum number (87.89%) of shoots obtained
from the micro-shoot culture method and a maximum number (91.12%) of roots were
obtained on MS medium supplemented with NAA (0.2 mg L-1), IBA (0.2
mgL-1) and IAA (0.1 mgL-1) which was found to be the
best. The healthy rooted plantlets were successfully transferred and obtained
as the plants were of 100% survival. The regenerated plants were
morphologically similar to the mother plant.
Author(s) Details
B. Chandra Sekhar Singh
Department of Plant Science,
College of Agriculture and Veterinary Sciences, Ambo University, Ambo, Post Box:
19, Ethiopia.
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