Skip to main content

Studies on Optimization of Papain Hydrolysis Conditions for Release of Glycosaminoglycans from the Chicken Keel Cartilage | Chapter 12 | New Visions in Science and Technology Vol. 1

The goal of this study was to find the best papain hydrolysis conditions for getting glycosaminoglycans from chicken keel cartilages (pH, E/S ratio, incubation temperature, and hydrolysis time). Glycosaminoglycans (GAGs) are natural biocompounds found in sharks, pigs, cows, chickens, and other animals that combine to generate cartilage tissuses. They can be extracted from shark, pig, cow, chicken, and other animal cartilage. GAGs contain chondroitin sulphate (CS), a functional food supplement that is used to prevent and treat arthritis and eye disorders. As a result, GAGs were extracted from byproducts of the cattle and poultry slaughter industries in order to determine the CS cont. Response surface approach was used to find the best hydrolysis conditions in this investigation (RSM). pH (x1), enzyme concentration (x2), incubation temperature (x3), and hydrolysis duration were the independent variables (x4). The variables had a substantial impact on the content of GAGs, according to the results of the analysis of variance (ANOVA). pH 7.1, 0.62 percent enzyme per substance w/wpo, 65°C, and a hydrolysis period of 230 minutes were found to be the optimal hydrolysis conditions, with GAG content reaching 14.3 percent of the dry matter of the raw material. According to HPLC analysis, 56.17 percent of the dry preparations of GAGs, which were made up of CS compounds, accounted for 8.11 percent of the dry matter in chicken keel cartilage. The dried GAGs preparations had a molecular weight of 259.6 kDa. Moisture 12.2 percent, protein 8.42 percent, fat 0 percent, ash 10.03 percent, and extracted GAGs 69.35 percent made up the dry preparations. Papain was utilised to hydrolyze chicken keel cartilage in this study. RSM used Mode 5.0 software to tune the hydrolysis conditions. This article helps to create a product that is healthy, high-quality, and low-cost.

 

Author (S) Details

Nguyen Thi Le Vien
Faculty of Chemical Engineering, Department of Food Technology, Ho Chi Minh City University of Technology, 268 Ly Thuong Kiet Street, District 10th, Vietnam and Vietnam National University Ho Chi Minh city, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam.

Pham Bao Nguyen
Center for Post-Harvest Technology, School of Agriculture and Aquaculture, Tra Vinh University, 126 Nguyen Thien Thanh, Ward 5, Tra Vinh, Vietnam.

Lam Duc Cuong
Faculty of Chemical Engineering, Department of Food Technology, Ho Chi Minh City University of Technology, 268 Ly Thuong Kiet Street, District 10th, Vietnam and Vietnam National University Ho Chi Minh city, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam.

Trinh Thi Thua An
Faculty of Chemical Engineering, Department of Food Technology, Ho Chi Minh City University of Technology, 268 Ly Thuong Kiet Street, District 10th, Vietnam and Vietnam National University Ho Chi Minh city, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam.

Dong Thi Anh Dao
Faculty of Chemical Engineering, Department of Food Technology, Ho Chi Minh City University of Technology, 268 Ly Thuong Kiet Street, District 10th, Vietnam and Vietnam National University Ho Chi Minh city, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam.

View Book :- https://stm.bookpi.org/NVST-V1/article/view/3210
Labels:

Comments

Post a Comment

Popular posts from this blog

Greening Regional Airports: A Vision for Carbon Neutral Infrastructure | Chapter 12 | Contemporary Perspective on Science, Technology and Research Vol. 3

 This study provides an overview of the energy demand of a regional airport, divided into individual time horizons. The electrification of aircraft systems raises the question of whether airports will be among the largest electricity consumers in our infrastructure in the future. Sustainability and especially emission reductions are significant challenges for airports that are currently being addressed. The Clean Sky 2 project GENESIS addresses the environmental sustainability of hybrid-electric 50-passenger aircraft systems in a life cycle perspective to support the development of a technology roadmap for the transition to sustainable and competitive electric aircraft systems. This article originates from the GENESIS research and describes various options for ground power supply at a regional airport. Potential solutions for airport infrastructure with a short (2030), medium (2040), and long (2050) time horizon are proposed. In addition to the environmental and conservation benefi...
Post a Comment
Read more

Alkali Element Modification of Glucose Molecules as a Method to Dissolve Cancer Cells | Chapter 12 | New Visions in Medicine and Medical Science Vol. 4

  The present study highlights about alkali element modification of glucose molecules as a method to dissolve cancer cells. The central regulation of the mechanisms governing cell proliferation has little effect on cancer cells. Cancer cells are entirely independent of the central command and divide and proliferate on their own, making it challenging to activate their response mechanism. Precisely, this is the reason why they are at risk to the health of humans and/or any biological entities. Instead of trying to reconnect the central command of the growth control mechanism to cancer cells that are already out of the range, we present a method of using the cancer cell’s own irresponsive and uncontrolled growth mechanism to their disadvantage and destroy the cancer cells. We found that this is achievable in an atomic/molecular level study of the glucose molecule, which is the primary food source used for growth and energy generation by all cells in the body, including the cancer cel...
Post a Comment
Read more

Diagnosis and Management of Scrapie in Post Mortem Goats by Immunohistochemistry | Chapter 6 | New Visions in Medicine and Medical Science Vol. 4

  The main objective of this study was to contribute to the annual passive surveillance for this pathology, since the goats are a species considered reservoir for the prion. A class of pathologies known as Transmissible Spongiform Illnesses (TSEs), which affect both humans and animals, includes scrapie, a neurological disease with a slow and deadly course that affects sheep, goats, and mouflons. Given that Immunohistochemistry (IHC) is the globally accepted technique for identifying classical scrapie in goats—a species thought to be a potential zoonotic disease reservoir. Chile in relation to Bovine Spongiform Encephalopathy (BSE), is classified as a disease-free country. Scrapie is actively monitored for the sheep, but passively for the caprine species, which is why this study was carried out in slaughtered goats in the central north of the country (IV Region), to provide information to the surveillance system for this disease. Fifty brains of goats older than 2 years were used, r...
Post a Comment
Read more