Skip to main content

Micropropagation of Narcissus tazetta ‘Chinensis’ and Its Relation to Secondary Metabolites Content: Brief Overview | Chapter 5 | Current Research Trends in Biological Science Vol. 1

In this study, bulbs of Narcissus tazetta ‘Chinensis’ were used as explants for micropropagation. Several disinfection protocols, callus medium and regeneration medium were tested in addition to the secondary metabolites content analysis. Four disinfection protocols were used. The most successful was: 1) disinfection of bulbs in 70% ethanol and 2% HgCl2, 10 minutes for each, outside cabinet, or 2) 70% ethyl alcohol (4 min), 20% clorox (5 min), 0.1% mercuric chloride (1 min) and 9% hydrogen peroxide (1 min) inside cabinet, washing with sterilized distilled water after every previous step. This technique is considered as a novel effective procedure. Indirect micropropagation on MS medium supplemented with 2 mg·L-1 2,4-D succeeded in narcissus callus induction which regenerated into shoots on MS medium supplemented with 1 mg.L-1 IBA + 1mg.L-1 TIBA + 1 gm.L-1 of activated charcoal. The same medium with activated charcoal sowed high yield in a direct micropropagation protocol. Average shoot and root per explant showed significant increase when using indirect micropropagation. Secondary metabolites from direct and indirect regeneration, using phytochemical screening detected increasing alkaloids content in callus only, while regenerated plantlets were the same as mother plants, as well as there were no significant changes in mother and regenerated plants in terms of total phenol and flavonoids content.
Author(s)Details

Hamdy M. Abdel-Rahman
Genetic Engineering and Biotechnology Division, Department of Genetics and Cytology, National Research Centre, 33 El Buhouth ST., 12622, Dokki, Giza, Egypt.

K. N. Rashed
Department of Pharmacognosy, National Research Centre, 33 El-Buhouth St.-Dokki, P.O. 12622, Giza, Egypt.

View Book: - http://bp.bookpi.org/index.php/bpi/catalog/book/157
Labels:

Comments

Post a Comment

Popular posts from this blog

Greening Regional Airports: A Vision for Carbon Neutral Infrastructure | Chapter 12 | Contemporary Perspective on Science, Technology and Research Vol. 3

 This study provides an overview of the energy demand of a regional airport, divided into individual time horizons. The electrification of aircraft systems raises the question of whether airports will be among the largest electricity consumers in our infrastructure in the future. Sustainability and especially emission reductions are significant challenges for airports that are currently being addressed. The Clean Sky 2 project GENESIS addresses the environmental sustainability of hybrid-electric 50-passenger aircraft systems in a life cycle perspective to support the development of a technology roadmap for the transition to sustainable and competitive electric aircraft systems. This article originates from the GENESIS research and describes various options for ground power supply at a regional airport. Potential solutions for airport infrastructure with a short (2030), medium (2040), and long (2050) time horizon are proposed. In addition to the environmental and conservation benefi...
Post a Comment
Read more

There is No Standard Model of ZFC and ZFC2 | Chapter 03 | Advances in Mathematics and Computer Science Vol. 1

In this Chapter we obtain a contradictions in formal set theories under assumption that these theories have omega-models or nonstandard model with standard part. An possible generalization of Lob’s theorem is considered. Main results are: (i) ¬Con(ZF C+ ∃ M ZFC st ), (ii) ¬Con(N F+ ∃ M NF st ), (iii) ¬Con(ZF C 2 ), (iv) let k be an inaccessible cardinal then ¬Con(ZF C+ ∃ κ), (v) ¬Con(ZF C+ (V=L)), (vi) ¬Con(ZF+ (V=L)). Author   Details: Jaykov Foukzon Israel Institute of Technology, Haifa, Israel Men'kova Elena Romanovna All-Russian Research Institute for Optical and Physical Measurements, Moscow, Russia Read full article: http://bp.bookpi.org/index.php/bpi/catalog/view/46/221/408-1 View Volume: https://doi.org/10.9734/bpi/amacs/v1
Post a Comment
Read more

Study on Processing Method Influences the Effect of Cassava (Manihot esculenta) Consumption on Blood Lipid Profile in Rats | Chapter 8 | Current Research and Development in Chemistry Vol.1

Cassava is consumed in many tropical countries and is fast becoming the major raw material of many diets, especially in the bakery, brewery and pharmaceutical industries. In a study to determine the effects of various formulations of Cassava feeds on blood lipid profile in rats. Forty adult Wistar rats weighing between 120 and 150 g were divided into 4 groups of 10 rats each. The rats were acclimatized for one week; fed with different formulations of Cassava feeds adlibitum, after which the rats were allotted into groups. Group 1 was fed on Garri; group 2 on Cassava flour; group 3 on Tapioca, and the control group (group 4) on normal chow. After 4 weeks, each animal was anaesthetized by exposure to chloroform vapour and blood samples collected by cardiac puncture. Group 1 diet (Garri) significantly increased HDL-cholesterol (HDL-C) concentration and significantly decreased LDL-cholesterol (LDL-C) concentration (p<0.05) compared with the control group. In Group 2 (Cassava flour), t...
Post a Comment
Read more