In vitro Impact Assessment of Aqueous Extract of Sida cordifolia Linn. Upon Rat Spermatozoa Parameters | Chapter 07 | Emerging Research in Medical Sciences Vol. 1
Sida cordifolia Linn. finds frequent
mention in Ayurvedic System of Medicine for many therapeutic properties
including beneficial effect upon male reproductive processes. Aim of present in
vitro study was to evaluate impact of aqueous extract of Sida cordifolia roots
on rat spermatozoa in terms of count, motility & morphology and through HOS
study following standard assessment methods to assess any likely reproductive
toxicity, spermicidal action, lethality or abnormalities in animal
experimentation. Sexually mature (18-22 weeks old) male Wister rats weighing
180 to 260 gm were used. Sperms were collected from cauda epididymis of rat
testes and sperm suspension was prepared by mixing them with 2 ml of 5% Sucrose
at 37°C, dividing into 4 samples – control (A) and 3 drug treated samples (B, C
& D) containing 10, 20 or 30 mg/ml of aqueous extract of Sida cordifolia.
The various sperm parameters were observed under Olympus CX41 microscope. Sperm
concentration, Progressive and Non-Progressive motility were calculated using
the Neubauer Chamber. The viability and morphology of sperm suspensions were
assessed using Eosin Y and Nigrosin staining method using 400 X magnification
under Phase contrast microscope. Hypo-osmotic swelling (HOS) test was used to
evaluate the functional integrity of plasma membrane. Treatment with aqueous
extract of Sida cordifolia resulted in no significant adverse impact upon sperm
count up to 30 mg/ml dose, suggesting no noticeable spermicidal effect.
Progressive motility increased marginally due to intervention of Sida
cordifolia in a concentration-dependent manner from 40.00±1.07 in Sample A to
43.50± 2.05 in Sample D. Similarly, percentage of normal sperms and their
viability exhibited steady enhancements with increasing concentrations of
research drug. The HOS test indicated no adverse structural changes in plasma
membrane integrity of sperms up to 30mg/ml level. Thus, in vitro assessment of
sperm motility, morphology and viability due to treatment with Sida cordifolia
aqueous extract indicated no spermicidal or toxic effect and indicated small
but significant enhancement in various parameters which was
concentration-dependent.
Author(s) Details
Dr. Mradu Gupta
Institute of Post Graduate
Ayurvedic Education and Research, 294/3/1, A. P. C. Road, Kolkata, 700009,
India.
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