In vitro Seed Germination Behaviour of Simmondsia chinensis | Chapter 15 | Modern Research in Botany Vol. 1
Simmondsia chinensis (Link) Schneider
(Simmondsiaceae) is a evergreen shrub of desert and marginal land of India. It
produces a unique liquid-wax which has commercial potential in pharmaceutical
industry. It is difficult to propagate vegetatively as it is a seasonal
procedure and grows slowly. Therefore, there is a need to improve the
propagation method through seed germination and seedling behaviour. The present
investigation was carried out to assess viability and in vitro germination
tests of fresh and old seeds. It was found that light (16/8-h day/night photoperiod)
conditions resulted in early and higher seed germination percentage as compared
to total dark period under in vitro culture conditions. Improvement in
germination percentage of one-year-old seeds was observed using Gibberellic
acid (GA3), Benzylaminopurine (BAP) and Thidiazuron (TDZ) under
given photoperiod conditions. Interestingly, TDZ 1.0 mg/L-1 were
produced highest germination rate of seeds (92%), as well as better seedling
growth, followed by BAP with 70% germination rate at 0.5 mg•L-1
concentration. The method of propagation through in vitro seed germination
could be effectively employed for large scale production of plant material.
Author(s) Details
Dr. Raman Bala
Department of Environmental
Science, Maharshi Dayanand University, Rohtak, India.
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