Antibacterial Activity of Flavonoids Extracted from Seeds of Pongamia pinnata Linn against Methicillin Resistant Staphylococcus aureus | Chapter 08 | Recent Advances in Biological Research Vol. 3
Introduction:
Methicillin resistant Staphylococcus aureus (MRSA) is defined as the resistant
to penicillinase-stable penicillin’s, thus the acronym MRSA is still under use
even though methicillin is no longer the agent of choice for treatment. The use
of vancomycin for MRSA remains as the treatment of choice but concerns with
rising resistance to glycol peptides call for the restrictive use of these
drugs. The resistance mechanism and the genes that mediate resistance have
presumably evolved in organism that produce antibiotics such that the
antibiotic produced is not effective against the producing organism.
Aims:
To assess the antibacterial property of seed crude
extracts of Pongamia pinnata Linn and isolated flavonoids component from crude
extract against Methicillin resistant Staphylococcus aureus obtained from
clinical isolates.
Study
Design: Observational study.
Place
and Duration of the Study: Department of Allied health sciences,
Department of Biochemistry and Department of Microbiology in Sri Devaraj Urs
Academy Of Higher Education and
Research, Tamaka, Kolar, between February 2014 and march 2015.
Methodology:
Confirmed clinical isolates for MRSA were collected from Microbiology
department to test the efficacy of crude extracts of seeds from Pongamia
pinnata L. Methanolic crude extract has been preferably used for isolation of
flavonoid content using Dimethyl Sulfoxide [DMSO] and methanol as ideal
solvents during extraction process by column chromatography technique. Agar
well diffusion method was performed to determine the antibacterial activity of
crude seed extracts of Pongamia pinnata and isolated flavonoids by using
quercitin as positive control for flavonoids. Vancomycin a glycopeptide powder
used as gold standard for comparing bactericidal activity of quercitin,
flavonoids and crude extracts of P. pinnata on MRSA.
Results:
The highest antibacterial activity (75-89%) was observed in crude extract of
Pongamia pinnata in comparison to vancomycin considered as cent percent.
Extracted flavonoids showed activity (66-92%) with respect to crude extract and
(50-84%) with vancomycin and the activity (71-92%) with respect to quercitin
when tested with concentration ranging from 25-400 µg/ml.
Conclusion:
This study showed that seed extracts of Pongamia pinnata L and its
phytochemical compound flavonoids showed potential antibacterial activity
against MRSA using quercitin and vancomycin. Flavonoids occupy the first grade
antimicrobials in combating methicillin resistant staphylococcus aureus
infections. These infections which are prominent in ICU units and HICU units
can be drastically controlled without any side effects.
Author Details:
Mary Shobha Rani Inala
Department
of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher
Education and Research, Kolar, Karnataka, India.
C. D. Dayanand
Department
of Biochemistry/Allied Health Sciences, Sri Devaraj Urs Academy of Higher
Education and Research, Kolar, Karnataka, India.
P. M. Beena
Department
of Microbiology, Sri Devaraj Urs Academy of Higher Education and Research,
Kolar, Karnataka, India.
A. V. M. Kutty
Department
of Biochemistry/Allied Health Sciences, Sri Devaraj Urs Academy of Higher
Education and Research, Kolar, Karnataka, India.
View Volume: https://doi.org/10.9734/bpi/rabr/v3
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