Skip to main content

Antibacterial Activity of Flavonoids Extracted from Seeds of Pongamia pinnata Linn against Methicillin Resistant Staphylococcus aureus | Chapter 08 | Recent Advances in Biological Research Vol. 3

Introduction: Methicillin resistant Staphylococcus aureus (MRSA) is defined as the resistant to penicillinase-stable penicillin’s, thus the acronym MRSA is still under use even though methicillin is no longer the agent of choice for treatment. The use of vancomycin for MRSA remains as the treatment of choice but concerns with rising resistance to glycol peptides call for the restrictive use of these drugs. The resistance mechanism and the genes that mediate resistance have presumably evolved in organism that produce antibiotics such that the antibiotic produced is not effective against the producing organism.

Aims: To assess the antibacterial property of seed crude extracts of Pongamia pinnata Linn and isolated flavonoids component from crude extract against Methicillin resistant Staphylococcus aureus obtained from clinical isolates.

Study Design: Observational study.

Place and Duration of the Study: Department of Allied health sciences, Department of Biochemistry and Department of Microbiology in Sri Devaraj Urs Academy Of Higher Education and  Research, Tamaka, Kolar, between February 2014 and march 2015.

Methodology: Confirmed clinical isolates for MRSA were collected from Microbiology department to test the efficacy of crude extracts of seeds from Pongamia pinnata L. Methanolic crude extract has been preferably used for isolation of flavonoid content using Dimethyl Sulfoxide [DMSO] and methanol as ideal solvents during extraction process by column chromatography technique. Agar well diffusion method was performed to determine the antibacterial activity of crude seed extracts of Pongamia pinnata and isolated flavonoids by using quercitin as positive control for flavonoids. Vancomycin a glycopeptide powder used as gold standard for comparing bactericidal activity of quercitin, flavonoids and crude extracts of P. pinnata on MRSA.

Results: The highest antibacterial activity (75-89%) was observed in crude extract of Pongamia pinnata in comparison to vancomycin considered as cent percent. Extracted flavonoids showed activity (66-92%) with respect to crude extract and (50-84%) with vancomycin and the activity (71-92%) with respect to quercitin when tested with concentration ranging from 25-400 µg/ml.

Conclusion: This study showed that seed extracts of Pongamia pinnata L and its phytochemical compound flavonoids showed potential antibacterial activity against MRSA using quercitin and vancomycin. Flavonoids occupy the first grade antimicrobials in combating methicillin resistant staphylococcus aureus infections. These infections which are prominent in ICU units and HICU units can be drastically controlled without any side effects.

Author  Details:

Mary Shobha Rani Inala
Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research, Kolar, Karnataka, India.

C. D. Dayanand
Department of Biochemistry/Allied Health Sciences, Sri Devaraj Urs Academy of Higher Education and Research, Kolar, Karnataka, India.

P. M. Beena
Department of Microbiology, Sri Devaraj Urs Academy of Higher Education and Research, Kolar, Karnataka, India.

A. V. M. Kutty
Department of Biochemistry/Allied Health Sciences, Sri Devaraj Urs Academy of Higher Education and Research, Kolar, Karnataka, India.


Comments

Popular posts from this blog

Empowerment and Leader Member Exchange towards Organizational Citizenship Behavior Readiness among Government Workforce in Malaysia | Book Publisher International

  State agencies are among the most significant entities that represent the public's needs, and their programmes are essential to a state's and the nation's economic growth and development. State agencies have been rife by public concerns for years, as demonstrated by the Public Complaints Bureau, as problems of inefficiency in service quality and delivery have yet to meet the public's standards. With the support of 15 employees from state agencies on Malaysia's east coast, this study aims to achieve three targets. The first and second objectives are to investigate the ties between leader-member exchange (LMX) and OCB empowerment. The final aim is to define the most significant factors that affect OCB willingness. A total of 288 people took part in the survey, which was conducted using a cluster sampling process. The survey items had achieved the necessary reliability, according to the pilot test results, and some sub-dimensions were renamed after factor analysis.

The Utilization of Agro-Waste: A Nanobiotechnology Point of View | Chapter 10 | Recent Advances in Biological Research Vol. 5

Aims: To review the utilization of agro-waste in the eco-friendly synthesis of nanoparticles and their biomedical, catalytic and industrial applications. Study Design: A review. Place and Duration of Study: This review was carried out in the interim of three weeks exploiting all relevant data, literature and publications where necessary. Methodology: Profound gathering of literatures/publications and reviews were employed with all carefulness and professional courtesy; enough useful information were gathered over time. The introduction emphasizes the dawn of science and technology, when great ideas were still latent later leading to the advent of history changing innovations like the completion of human genome and then the birth of nanotechnology. Although, nanotechnology had been known decades been popularized by Richard Feynman in his talks in the year 1959; and in this present dispensation, nanotechnology has been a solution to many intricate challenges/ t

Re-asserting Cultural Perspectives: Old People and New Ideas in Bole Butake’s Lake God and The Survivors and Sankie Maimo’s Succession in Sarkov | Chapter 10 | Perspectives of Arts and Social Studies Vol. 2

African cultures have undergone transformations from the colonial period to the present, often to the detriment of its cultural evolution and within the larger global community. As it were, colonial education subordinated African communalism and created in its place an anti-African spirit evident in the assimilation of western values. This did not only end up in a kind of cultural betrayal, but also posed as a serious threat to the dignity and identity of the people. Those who fall prey to this kind of cultural imperialism are the young people who are often irrationally carried away by western fashion and modes that they tend to neglect and/or forget their cultural ways of life as they join the race of “progress.” This situation has given rise to a conscious effort by creative writers to re-assert and protect African values while at the same time liberating themselves from the longstanding western effort at suppressing, controlling and dominating their thoughts particularly through n