Skip to main content

Antibiotics Susceptibility Pattern of Methicillin Resistant Staphylococcus aureus (MRSA) in Enugu State, South-East Region of Nigeria | Chapter 08 | Recent Advances in Biological Research Vol. 2

Development of antimicrobial resistance by bacteria is now a worldwide health issue, as infection is one of the leading causes of death in the world today. The aim of this study was to evaluate the prevalence and antimicrobials susceptibility pattern of Methicilin-Resistant Staphylococcus aureus in 3 different hospitals in South-East geopolitical region of Nigeria. The identification and confirmation of the S. aureus were done using selective and differential medium (Mannitol salt agar) for S. aureus and by coagulase/staphylase test using Oxoid® reagents kits (DR0595A). The method used for antibiotics susceptibility pattern of the characterised S. aureus isolates was discs diffusion method, as recommended by the Clinical Laboratory Standards Institute (CLSI), discs containing oxacillin (5 µg/disc), vancomycin (30 µg/disc), cephalexin (30 µg/disc), levofloxacin (5 µg/disc), ciprofloxacin (5 µg/disc), tetracycline (30 µg/disc), cotrimoxazole (25 µg/disc), gentamicin (30 µg/disc), clindamycin (2 µg/disc) and rifampicin (5 µg/disc). MRSA confirmation was done using Oxoid® DR0900 penicillin binding protein (pbp2’) latex agglutination test kits. The results showed that out of 218 characterized clinical isolates, 39 of it were confirmed MRSA with varying percentages of resistance to various antibiotics thus: oxacillin (62.07%), vancomycin (60.35%), cephalexin (55.18%), levofloxacin (56.90%), ciprofloxacin (65.52%), tetracycline (68.97%), cotrimoxazole (67.25%), gentamicin (62.07%), clindamycin (63.79%) and rifampicin (62.07%). The S. aureus are more sensitive to Levofloxacin and less sensitive to tetracycline, clindamycin and rimfapicin. Latex agglutination test confirmed 39 strains of the clinical isolates to be MRSA. The results shows open wound as a source with highest prevalence and sputum with lowest prevalence of the MRSA with no significant change (P > 0.05).

Biography of author(s)

A. A. Agboke
Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, University of Uyo, Uyo, Akwa Ibom State, Nigeria.

A. A. Attama
Departments of Pharmaceutics and Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, University of Nigeria, Enugu State, Nigeria.

Read full article: http://bp.bookpi.org/index.php/bpi/catalog/view/45/212/366-1

View Volume: https://doi.org/10.9734/bpi/rabr/v2
Labels:

Comments

Post a Comment

Popular posts from this blog

A Prospective Study about Safety and Efficacy of Perioperative Lidocaine Infusion | Chapter 09 | New Horizons in Medicine and Medical Research Vol. 8

 Opioids cause clinically significant side effects such as respiratory depression, immunosuppression, muscle rigidity, negative inotropism, nausea, vomiting, hyperalgesia, urine retention, postoperative ileus, and drowsiness. Perioperative opioids are a major contributor to the United States' and other countries' opioid epidemics. Non-opioid analgesics, particularly lidocaine, are becoming more common for perioperative use as a result of this. A total of 185 adult patients were randomly assigned to one of two groups: control group I (105 patients) [fentanyl group] or group ii (80 patients) [opioid-free anaesthesia group]. Lidocaine 1.5 mg/kg bolus followed by 1.5 mg/kg/h infusion intraoperatively, and 1.5-2 mg/kg/h infusion for 2-8 hours postoperatively were given to patients in both groups at anaesthetic induction. Intraoperatively, both groups received analgesic adjuvants such as diclofenac 75 mg, paracetamol 1 gm, and mgso4 30-50 mg/kg. If the mean arterial pressure (map)
Post a Comment
Read more

A Brief Study of Middleware Technologies: Programming Applications and Management Systems | Chapter 15 | Novel Research Aspects in Mathematical and Computer Science Vol. 1

  Many platforms, services, applications, hardware, and operating systems are connected through the middleware layer. Because the middleware layer abstracts much low-level complexity and makes applications and software systems portable, it allows disparate systems to interface and function together in harmony. Middleware technologies enable software engineers to swiftly construct software systems and applications, allowing developers to focus on more important tasks. This chapter examines several types of middleware systems and discusses middleware capabilities, middleware operation, middleware's function in cloud-based systems, and the best middleware platforms to use. Middleware systems are widely utilised and can be found in practically any software system or application. Middleware programmes provide as a link between many sorts of systems and protocols. They serve as a mechanism for various systems. To successfully exchange information, it runs on a variety of operating system
Post a Comment
Read more

Patients’ Perspective of Acute Post-operative Pain Management: A Multicentre Survey of Tertiary Hospitals in Maharashtra, India | Chapter 08 | New Horizons in Medicine and Medical Research Vol. 8

 When postoperative pain is adequately controlled, patients' satisfaction and patient-related outcomes (PROs) increase. Understanding the patients' perspective is crucial since it supports in the formulation of improvement strategies. Because wrong attitudes and assumptions might block pain alleviation, patients' attitudes and beliefs are critical. As a result, a multicenter study of patients' attitudes, beliefs, experiences, and satisfaction levels with acute postoperative pain management was done in Maharashtra's tertiary hospitals. In addition, the responses were examined to evaluate if the Acute Pain Service (APS) resulted in improved patient outcomes and satisfaction. A 13-item questionnaire adapted from previous studies was used to capture patients' experiences with postoperative pain treatment. The responses of 179 patients are included in the study. The findings revealed that 91.6 percent of patients experienced postoperative pain, with 75.5 percent
Post a Comment
Read more