Bacillus spp with an Antibacterial Activity | Chapter 13 | Recent Advances in Biological Research Vol. 2
Bacterial resistance to antibiotics
poses a serious challenge to chemotherapy. Indeed, in recent decades, the risk
of contracting infectious diseases has increased significantly due to the
emergence and spread of multi-drug-resistant bacteria. The whole world is thus
confronted with this situation which motivated scientists to find out new
active molecules with an antibacterial effect. Therefore, the present
investigation aims to isolate and identify microorganisms secreting substances
with an antibacterial activity and to partially characterize their extracts.
Thus, six bacteria were isolated from soil and water of different biotopes of
Fez Morocco having an antibacterial effect against M. smegmatis, M. aurum,
Gram-positive and Gram-negative bacteria. Based on biochemical characterization
and 16S rDNA sequence analysis, it was revealed that the isolates belong to the
genus Bacillus. The antibacterial activity of three of them was fully affected
by proteases (pepsin and proteinase K) and heat treatment at 80ºC and 100ºC but
it was stable at 4ºC for a month and 37ºC during 3h. The lost of the activity
suggests a proteinaceous nature of the bio-active compounds, which might be
useful in the development of antibacterial agents after their total
purification in further work against bacterial infections.
Biography of author(s)
Dr. Ilham Zahir
Laboratory of Microbial
Biotechnology, Department of Biology, Faculty of Sciences and Technical,
University Sidi Mohamed Ben Abdellah, BP 2202, Road of Immouzer, Fez, Morocco
and Polyvalent Laboratory in Research and Development, Department of Biology,
Polydisciplinary Faculty, Sultan Moulay Slimane University, Beni Mellal,
Morocco.
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