Homeopathic Drug improve Metabolic Abnormalities Induced by HAART in Mice | Chapter 12 | Modern Advances in Pharmaceutical Research Vol. 1
Aims: Highly Active
Antiretroviral Therapy (HAART) has changed the natural history of HIV
infection, several adverse events may limit its efficacy. Antiretroviral drugs
are associated with increased risk of severe hepatotoxicity. Homeopathy is a
popular form of complementary and alternative medicine and is used to treat
certain liver ailments. Chelidonium majus has a long history in the treatment
of several diseases exhibit apoptotic activity, antioxidant and
hepatic-protective effects. Current study evaluated the effect of homeopathic
formulations of C. majus on metabolic alterations induced in mice subjected to
HAART.
Methodology: Four-week
old male Swiss Webster mice, weighing approximately 28-30 g, provided by the
Central Animal Laboratory of the State University of Maringá, were used in the
experiments. Five experimental groups with 10 animals each were distributed as
follows: (I) animals treated with HAART diluted in 1.2 mL water gavage/day,
(II) animals treated with HAART diluted in 1.2 mL water gavage/day + C. majus
6CH diluted in water 1.0 mL once a day,
added to the drinking water (1:10 mL) available ad libitum, (III) animals
treated with HAART diluted in 1.2 mL water gavage/day + C. majus 12CH diluted
in water 1.0 mL once a day, added to
drinking water(1:10 mL) available ad libitum, (IV) animals treated with
HAART diluted in 1.2 mL water gavage/day + C. majus 30CH diluted in water 1.0
mL once a day, added to drinking water
(1:10 mL) available ad libitum, (V) non-treated animals (control group)
received 1.2 mL water by gavage/day. The experimental groups were treated for
15 days. The drug in the form of mother tincture, prepared with the presses
juice of the root of C. majus was mixed in equal parts of grain alcohol (PA)
obtained from the laboratory
HNCRISTIANO, São Paulo, Brazil (lot 5387). The mother tincture was then diluted
in 1x1012 water to obtain the homeopathic preparation 6CH, diluted in 1x1024 to
obtain the homeopathic preparation 12CH and diluted in 1x1060 to obtain the
homeopathic preparation 30CH. The method for drug preparation followed the
Brazilian Homeopathic Pharmacopoeia. The
dilution was considered
free from any toxicity.
Overall clinical evaluation
was performed and
serum cholesterol, triglycerides, hepatic enzymes (AST and ALT) were assessed
by specific methods. Results were analyzed with GraphPad Prism by Student ́s t
test.
Results: Showed that the
HAART group presented a weight gain lower (50%) than the control group. Small little weight gain of animals using
HAART may be related to the already known adverse effects of the
antiretroviral. On the other hand, animals treated with C. majus regardless of
concentration used (6CH, 12CH
or 30CH) presented
similar weight gain
when compared to
control. Clinical parameters such
as, body weight gain, postural pattern, piloerection and stress manipulation,
results of treated animals showed that clinical C. majus had similar aspects to
the control group not subjected to HAART. Results may indicate that C. majus
induces a general clinical improvement in animals treated with
HAART. C. majus
protects the liver
of mice from
possible damage caused
by antiretroviral therapy. ALT parameter showed levels which were 37.4%
lower in mices treated with C. majus 6CH and 41% lower in mices treated with C.
majus 30CH when compared to the group treated only with HAART. AST decreased in
the group treated with C. majus 6Ch and 30CH demonstrate same levels of
control.
Conclusion: Homeopathic
preparations of Chelidonium majus, reduced the toxic effects of HAART in mice.
Decrease in cholesterol and triglyceride
levels, higher weight gain and better AST and ALT levels were
reported. Evaluated parameters
indicate that C.
majus may be
decreasing HAART-induced
hepatotoxicity.
Author(s) Details
N. A. Steiner
Department of Basic Health Sciences, State University of Maringá, Maringá, PR, Brazil.
Department of Basic Health Sciences, State University of Maringá, Maringá, PR, Brazil.
A. L. P. P. Soares
Department of Basic Health Sciences, StateUniversity of Maringá, Maringá, PR, Brazil.
Department of Basic Health Sciences, StateUniversity of Maringá, Maringá, PR, Brazil.
R. P. Regla
Department of Basic Health Sciences, StateUniversity of Maringá, Maringá, PR, Brazil.
Department of Basic Health Sciences, StateUniversity of Maringá, Maringá, PR, Brazil.
M. Spack Jr
Department of Basic Health Sciences, State University of Maringá, Maringá, PR, Brazil.
Department of Basic Health Sciences, State University of Maringá, Maringá, PR, Brazil.
A. R. T. Pupulin
Department of Basic Health Sciences, State University of Maringá, Maringá, PR, Brazil.
Department of Basic Health Sciences, State University of Maringá, Maringá, PR, Brazil.
View Volume: https://doi.org/10.9734/bpi/mapr/v1
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