Skip to main content

Homeopathic Drug improve Metabolic Abnormalities Induced by HAART in Mice | Chapter 12 | Modern Advances in Pharmaceutical Research Vol. 1

Aims: Highly Active Antiretroviral Therapy (HAART) has changed the natural history of HIV infection, several adverse events may limit its efficacy. Antiretroviral drugs are associated with increased risk of severe hepatotoxicity. Homeopathy is a popular form of complementary and alternative medicine and is used to treat certain liver ailments. Chelidonium majus has a long history in the treatment of several diseases exhibit apoptotic activity, antioxidant and hepatic-protective effects. Current study evaluated the effect of homeopathic formulations of C. majus on metabolic alterations induced in mice subjected to HAART.
Methodology: Four-week old male Swiss Webster mice, weighing approximately 28-30 g, provided by the Central Animal Laboratory of the State University of Maringá, were used in the experiments. Five experimental groups with 10 animals each were distributed as follows: (I) animals treated with HAART diluted in 1.2 mL water gavage/day, (II) animals treated with HAART diluted in 1.2 mL water gavage/day + C. majus 6CH diluted  in water 1.0 mL once a day, added to the drinking water (1:10 mL) available ad libitum, (III) animals treated with HAART diluted in 1.2 mL water gavage/day + C. majus 12CH diluted in water 1.0 mL once a day, added to  drinking water(1:10 mL) available ad libitum, (IV) animals treated with HAART diluted in 1.2 mL water gavage/day + C. majus 30CH diluted in water 1.0 mL once a day, added to  drinking water (1:10 mL) available ad libitum, (V) non-treated animals (control group) received 1.2 mL water by gavage/day. The experimental groups were treated for 15 days. The drug in the form of mother tincture, prepared with the presses juice of the root of C. majus was mixed in equal parts of grain alcohol (PA) obtained from the  laboratory HNCRISTIANO, São Paulo, Brazil (lot 5387). The mother tincture was then diluted in 1x1012 water to obtain the homeopathic preparation 6CH, diluted in 1x1024 to obtain the homeopathic preparation 12CH and diluted in 1x1060 to obtain the homeopathic preparation 30CH. The method for drug preparation followed  the  Brazilian  Homeopathic  Pharmacopoeia.  The  dilution  was  considered  free  from  any toxicity.  Overall  clinical  evaluation  was  performed  and  serum  cholesterol,  triglycerides,  hepatic enzymes (AST and ALT) were assessed by specific methods. Results were analyzed with GraphPad Prism by Student ́s t test.
Results: Showed that the HAART group presented a weight gain lower (50%) than the control group.  Small little weight gain of animals using HAART may be related to the already known adverse effects of the antiretroviral. On the other hand, animals treated with C. majus regardless of concentration used  (6CH,  12CH  or  30CH)  presented  similar  weight  gain  when  compared  to  control.  Clinical parameters such as, body weight gain, postural pattern, piloerection and stress manipulation, results of treated animals showed that clinical C. majus had similar aspects to the control group not subjected to HAART. Results may indicate that C. majus induces a general clinical improvement in animals treated  with  HAART.  C.  majus  protects  the  liver  of  mice  from  possible  damage  caused  by antiretroviral therapy. ALT parameter showed levels which were 37.4% lower in mices treated with C. majus 6CH and 41% lower in mices treated with C. majus 30CH when compared to the group treated only with HAART. AST decreased in the group treated with C. majus 6Ch and 30CH demonstrate same levels of control.
Conclusion: Homeopathic preparations of Chelidonium majus, reduced the toxic effects of HAART in mice. Decrease in cholesterol  and triglyceride levels, higher weight gain and better AST and ALT levels  were  reported.  Evaluated  parameters  indicate  that  C.  majus  may  be  decreasing  HAART-induced hepatotoxicity.

Author(s) Details

N. A. Steiner
Department of Basic Health Sciences, State University of Maringá, Maringá, PR, Brazil.
A. L. P. P. Soares
Department of Basic Health Sciences, StateUniversity of Maringá, Maringá, PR, Brazil.
R. P. Regla
Department of Basic Health Sciences, StateUniversity of Maringá, Maringá, PR, Brazil.
M. Spack Jr
Department of Basic Health Sciences, State University of Maringá, Maringá, PR, Brazil.
A. R. T. Pupulin
Department of Basic Health Sciences, State University of Maringá, Maringá, PR, Brazil.

Comments

Popular posts from this blog

Empowerment and Leader Member Exchange towards Organizational Citizenship Behavior Readiness among Government Workforce in Malaysia | Book Publisher International

  State agencies are among the most significant entities that represent the public's needs, and their programmes are essential to a state's and the nation's economic growth and development. State agencies have been rife by public concerns for years, as demonstrated by the Public Complaints Bureau, as problems of inefficiency in service quality and delivery have yet to meet the public's standards. With the support of 15 employees from state agencies on Malaysia's east coast, this study aims to achieve three targets. The first and second objectives are to investigate the ties between leader-member exchange (LMX) and OCB empowerment. The final aim is to define the most significant factors that affect OCB willingness. A total of 288 people took part in the survey, which was conducted using a cluster sampling process. The survey items had achieved the necessary reliability, according to the pilot test results, and some sub-dimensions were renamed after factor analysis.

Re-asserting Cultural Perspectives: Old People and New Ideas in Bole Butake’s Lake God and The Survivors and Sankie Maimo’s Succession in Sarkov | Chapter 10 | Perspectives of Arts and Social Studies Vol. 2

African cultures have undergone transformations from the colonial period to the present, often to the detriment of its cultural evolution and within the larger global community. As it were, colonial education subordinated African communalism and created in its place an anti-African spirit evident in the assimilation of western values. This did not only end up in a kind of cultural betrayal, but also posed as a serious threat to the dignity and identity of the people. Those who fall prey to this kind of cultural imperialism are the young people who are often irrationally carried away by western fashion and modes that they tend to neglect and/or forget their cultural ways of life as they join the race of “progress.” This situation has given rise to a conscious effort by creative writers to re-assert and protect African values while at the same time liberating themselves from the longstanding western effort at suppressing, controlling and dominating their thoughts particularly through n

The Utilization of Agro-Waste: A Nanobiotechnology Point of View | Chapter 10 | Recent Advances in Biological Research Vol. 5

Aims: To review the utilization of agro-waste in the eco-friendly synthesis of nanoparticles and their biomedical, catalytic and industrial applications. Study Design: A review. Place and Duration of Study: This review was carried out in the interim of three weeks exploiting all relevant data, literature and publications where necessary. Methodology: Profound gathering of literatures/publications and reviews were employed with all carefulness and professional courtesy; enough useful information were gathered over time. The introduction emphasizes the dawn of science and technology, when great ideas were still latent later leading to the advent of history changing innovations like the completion of human genome and then the birth of nanotechnology. Although, nanotechnology had been known decades been popularized by Richard Feynman in his talks in the year 1959; and in this present dispensation, nanotechnology has been a solution to many intricate challenges/ t