Isolation, Identification and Pathogenicity of Fungal Organisms Causing Postharvest Spoilage of Tomato Fruits during Storage | Chapter 06 | Recent Advances in Biological Research Vol. 3
Tomato is one of the most widely grown
and extensively consumed horticultural crops in the world. Isolation,
identification and pathogenicity of fungal organisms causing postharvest
spoilage of tomato fruits during storage was carried out. Tomato fruits showing
symptoms of rot were collected from the store house. Small sizes were cut and
surface sterilized in 1% of Sodium hydrochloride and rinsed in several changes
of sterile distilled water. They were plated on Potato Dextrose Agar (PDA) and
observed for fungal growth. Identification was done macroscopically and
microscopically. For pathogenicity, healthy tomato fruits were plugged with
pure cultures of the fungal isolates and disease incidence and severity were
evaluated. Five fungi namely Aspergillus flavus, Penicillium waksmanii,
Botryodiplodia theobromae, Fusarium oxysporum and Colletotrichum asianum were
isolated. Incidence of decay on healthy tomato fruits was 100% for all fungal
isolates while the control was 0%. T-test revealed significant differences
between the inoculated and the controls at 1% and 5% levels of probability.
Severity of decay ranged from 51–53% for all fungal isolates, while the
controls showed 0%. T-test revealed significant differences between the
inoculated and the control at 1% and 5% levels of probability. Pathogenic
microorganisms on tomato are a potential health hazard to man and animals
following ingestion.
Author Details:
Dr. Liamngee Kator
Department
of Biological Sciences, Benue State University, Makurdi, Benue State, Nigeria
A. C. Iheanacho
Department
of Agribusiness, University of Agriculture, Makurdi, Benue State, Nigeria.
Kortse P. Aloho
Department
of Plant Breeding and Seed Science, University of Agriculture, Makurdi, Benue
State, Nigeria.
View Volume: https://doi.org/10.9734/bpi/rabr/v3
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