Isolation of Rare Salmonella Serovars, Wangata and Penarth from Chicken in Nsukka, Nigeria | Chapter 03 | Recent Advances in Biological Research Vol. 3
Introduction:
Salmonella infections remain a veterinary and public health problem of major
importance. Poultry birds are known to be one of the major reservoirs of
Salmonella and could consequently act as a vehicular transmission route to
humans. Rare Salmonella serovars, whose epidemiological and serological
patterns are not well understood, are becoming increasingly common in Nigeria
and other parts of the world. We report the isolation of Salmonella enterica
serovars Wangata and Penarth, two serovars that had not been previously
reported in chicken in Nigeria.
Materials
and Methods: A total of 300 chickens comprising of
150 intensively reared and 150 free range chickens, from selected farms and
live bird markets, were sampled via cloacal vent using sterile cotton swab tips
according to the International Office of Epizootics (OIE) standards. Following
standard bacteriological techniques, samples were pre-enriched in buffered
peptone water, before transferring into Rappaport Vassiliadis medium and
finally streaked onto Salmonella-Shigella agar (SSA). Salmonella spp. were
identified biochemically and serotyped based on reaction with somatic (O),
flagella (H), and capsular (Vi) antisera. Antibiotic susceptibility testing was
performed following Kirby-Bauer (disk-diffusion) method.
Results:
Out of the 300 samples, 4% (n = 12) were positive for salmonellae. The isolates
comprise of 6 isolates of S. enterica ser Wangata, 5 S. enterica ser
Enteritidis and 1 S. enterica ser Penarth. All the rare serotypes S. Wangata
and S. Penarth were isolated from free range chickens, while S. Enteritidis was
isolated from both intensively reared and free range chickens. There was no
difference in the sensitivity pattern between the rare serovars and serovar
Enteritidis to the antibiotics tested. S. Penarth had a higher MIC to
Cotrimoxazole, but lower MBC for gentamicin and tetracycline.
Conclusions:
Free range chickens could be vehicles for the transmission and/or reservoirs of
the rare salmonellae serotypes in Nigeria. Any prophylactic program aimed at
controlling these agents in poultry farms in Nigeria, must take into account
the free range local chickens.
Author Details:
Obi, Okechukwu J.
Department
of Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria.
Ike, Anthony C.
Department
of Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria.
Olovo, Chinasa V.
Department
of Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria.
View Volume: https://doi.org/10.9734/bpi/rabr/v3
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