Physicochemical Properties of an Avian Protease Inhibitor | Chapter 06 | Recent Advances in Biological Research Vol. 2
Background:
Ovomucoid is a serine proteinase inhibitor in the egg whites of all avian
species at a concentration of about 10 mg/ml. The involvement of proteinases in
a multitude of control functions in an organism has created an interest in
their physiological inhibitors. Regulation of proteolytic activity in tissues
is a critical requirement in the maintenance of homeostasis. Egg white proteins
possess ACE-inhibitory activity & high radical-scavenging activity. The
combined antioxidant and ACE-inhibitory properties of egg white hydrolysates, or
the corresponding peptides would make a useful multifunctional preparation for
the control of cardiovascular diseases. Proteases play key roles in several
physiological processes, including intracellular protein degradation, bone
remodeling, and antigen presentation, and their activities are increased in
pathophysiological conditions such as, cancer metastasis and inflammation. They
are also required for invasion by the microorganism. Four protease inhibitors
have been identified in egg white namely, cystatin, ovomucoid, ovomacroglobulin
(also known as ovostatin), and ovoinhibitor. Use of proteinase inhibitors in
the treatment of certain diseases has renewed interest in their specificity and
stability, both of which in turn depend on the tertiary structure of the
inhibitor. Structural alteration to obtain molecules of desired properties
requires knowledge of relationship between structure, function and stability.
Aims:
Given its importance, in the present study duck ovomucoid was isolated and
characterized for its physicochemical properties.
Methodology: Duck ovomucoid was
isolated and characterized by its physicochemical properties. Analytical gel
filtration (Sephacryl S-100 HR column) was used for purification, determination
of molecular weight (MW), carbohydrate content and Stokes radius.
Results & Conclusion: The
fluorescence emission spectrum was 302 nm, comparable to earlier reports.
Stoke’s radius was found to be 2.91nm, and the value was comparable with white
leghorn hen (Stoke’s radius 3.15nm). The extraordinary large value of stokes
radius can be attributed to its high carbohydrate content which increases the
hydration of the molecule. The inhibitor had the molecular weight of 29,300,
and the carbohydrate content was 22%, the specific extinction coefficient of
duck ovomucoid was found to be 5.82 at 279 nm and the Stoke’s radius was
2.91nm. The extraordinary large value of stokes’ radius of duck ovomucoid can
be attributed to its high carbohydrate content which increases the hydration of
the molecule resulting in large Stoke’s radius. Further functional studies on
duck ovomucoid are required, as it has been reported that the chicken ovomucoid
hydrosylates have some exceptional antioxidant, ACE inhibitory and metal
chelating activities.
Biography of author(s)
Khushtar Anwar Salman
Department of Biochemistry,
JN Medical College, A M U Aligarh, India.
Sharique Ahmed
Department of Allied Health,
College of Health Sciences, University of Bahrain,Bahrain.
Indu Saxena
Department of Biochemistry,
AIIMS, Jodhpur, India.
View Volume: https://doi.org/10.9734/bpi/rabr/v2
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