Skip to main content

Reproducible Agrobacterium-mediated Transformation of Nigerian Cultivars of Tomato (Solanum lycopersicum L.) | Chapter 01 | Advances and Trends in Agricultural Sciences Vol. 1

This study was carried out to develop transformation protocol for the possible improvement of local cultivars of tomatoes in Nigeria using complete randomized design (CRD). The research was conducted at the Plant Biotechnology Centre, International Institute of Tropical Agriculture (IITA), Ibadan, Oyo State, Nigeria between May 2009 and December 2009. Seeds of three promising farmer preferred varieties of cultivars of tomatoes namely Ibadan local, Ife and JM94/46 were selected and cultivated in-vitro. Sterile cotyledon and leaf explants were transformed using Agrobacterium tumefaciens strain LBA4404 with plasmid (pOYE153). Transformed plants were analyzed using GUS assay and PCR methods. Results showed that leaf explants had higher transformation efficiency than cotyledon explants in the three cultivars. Ife cultivar had the best transformation efficiency in both explant types - leaf 42.5% and cotyledon 8.89%. Histochemical GUS assay of transgenic plants showed blue coloration in leaves, stems and roots. PCR analysis showed amplification of 600 bp fragments of GUS and nptII genes in the transgenic plants on 1.0% agarose gel. The GUS and nptII genes were successfully integrated into the three cultivars of tomatoes thereby providing a reliable transformation protocol for the genetic improvement of local cultivars of tomatoes for desirable traits such as longer shelf-life, pest and disease resistance, enhanced nutrients, higher soluble solids, etc. The GUS and nptII genes were successfully integrated into the three cultivars of tomatoes thereby providing a reliable transformation protocol for the genetic improvement of local cultivars of tomatoes for desirable traits such as longer shelf-life, pest and disease resistance, enhanced nutrients, higher soluble solids, etc.

Author(s) Details

Dr. S. O. A. Ajenifujah-Solebo
National Biotechnology Development Agency, Abuja, Nigeria.

Read full article: http://bp.bookpi.org/index.php/bpi/catalog/view/52/407/440-1
View Volume: https://doi.org/10.9734/bpi/atias/v1

Labels

Labels:

Comments

Post a Comment

Popular posts from this blog

Greening Regional Airports: A Vision for Carbon Neutral Infrastructure | Chapter 12 | Contemporary Perspective on Science, Technology and Research Vol. 3

 This study provides an overview of the energy demand of a regional airport, divided into individual time horizons. The electrification of aircraft systems raises the question of whether airports will be among the largest electricity consumers in our infrastructure in the future. Sustainability and especially emission reductions are significant challenges for airports that are currently being addressed. The Clean Sky 2 project GENESIS addresses the environmental sustainability of hybrid-electric 50-passenger aircraft systems in a life cycle perspective to support the development of a technology roadmap for the transition to sustainable and competitive electric aircraft systems. This article originates from the GENESIS research and describes various options for ground power supply at a regional airport. Potential solutions for airport infrastructure with a short (2030), medium (2040), and long (2050) time horizon are proposed. In addition to the environmental and conservation benefi...
Post a Comment
Read more

Occipital Dermal Sinus Tract Causing Craniospinal Infection: A Review | Chapter 13 | New Visions in Medicine and Medical Science Vol. 4

  Dermal sinus is a rare congenital condition characterized by a pathological tract connecting the skin to deeper tissues of the central nervous system, potentially leading to severe infectious complications. It arises from a failure in the separation of ectodermal layers during early gestation. Diagnosing dermal sinus tract in newborns requires a careful physical examination, focusing on midline dimples in the occipital region associated with cutaneous abnormalities like hairy nevus or hyperpigmentation. The presence of drainage, abnormal hair distribution, or localized swelling may indicate a sinus tract. Regular examination for dimples or sinuses is recommended for infants and children with recurrent meningitis or infections. Early detection is crucial to prevent severe complications like meningitis, with Staphylococcus aureus being a common causative organism. Neuroradiological studies, including computed tomography (CT) scan and magnetic resonance imaging (MRI), with histopath...
Post a Comment
Read more

Alkali Element Modification of Glucose Molecules as a Method to Dissolve Cancer Cells | Chapter 12 | New Visions in Medicine and Medical Science Vol. 4

  The present study highlights about alkali element modification of glucose molecules as a method to dissolve cancer cells. The central regulation of the mechanisms governing cell proliferation has little effect on cancer cells. Cancer cells are entirely independent of the central command and divide and proliferate on their own, making it challenging to activate their response mechanism. Precisely, this is the reason why they are at risk to the health of humans and/or any biological entities. Instead of trying to reconnect the central command of the growth control mechanism to cancer cells that are already out of the range, we present a method of using the cancer cell’s own irresponsive and uncontrolled growth mechanism to their disadvantage and destroy the cancer cells. We found that this is achievable in an atomic/molecular level study of the glucose molecule, which is the primary food source used for growth and energy generation by all cells in the body, including the cancer cel...
Post a Comment
Read more