Soil Bacteria as a Natural Source of Antifungal Agents against Phytopathogenic Fungus: Aspergillus niger | Chapter 01 | Recent Advances in Biological Research Vol. 2
Soil bacteria are able to synthesize a
wide range of metabolites with fungicidal activity. Nine bacterial isolates
were obtained from the botanical garden of University of Calabar. Preliminary
examination of isolates was carried out using morphological characteristics and
biochemical analysis. These isolates were designated with codes SB1, SB2, SB3, SB4, SB5, SB6, SB7, and SB8. Bacterial isolates
were evaluated for their potentials of antagonism against Aspergillus
niger isolated from spoiled vegetables like
tomatoes by using
agar diffusion technique.
Percentage inhibition of
mycelial growth by
these isolates recorded values
as 27%, 0%,
66%, 40%, 97%,
0% and 23%
respectively. Isolates were analyzed
through several biochemical
tests and were
identified as Bacillus sp., Enterobacter spp., Pseudomonas spp., Proteus
spp., Escherichia coli,
Streptococcus spp. and Staphylococcus spp.respectively. These
result indicated that
bacterial species exhibited
varying degree of
antagonism against the fungus Aspergillus niger. Escherichia coli
showed maximum inhibitory
potential against tested
fungus with reduction of up to 97% in
their mycelial fungal growth, followed by Pseudomonas spp. and Bacillus
spp. with 66.7%
reduction. From the
results obtained, it
is found that
the bacterial species isolated
from the soil
samples are effective
as antifungal agents
against phyto-pathogenic fungi.
Escherichia coli, Pseudomonas
spp., Bacillus spp.
had more antagonistic
effect on vegetative growth of
tested fungi (Aspergillus
niger). These bacterial
species showed higher
levels of inhibitory effect on
fungal spore germination.
The presented data
exhibit the antifungal
activity of bacterial species and indicate the
possibility of using these bacterial species as a biological agent to control
the phyto pathogenic fungi.
Biography of author(s)
Mrs. Anitha Prakash Rao
Department of Microbiology,
Faculty of Biological Sciences, University of Calabar, P.M.B. 1115, Calabar,
Nigeria.
Dr. Bassey Etta Agbo
Department of Microbiology,
Faculty of Biological Sciences, University of Calabar, P.M.B. 1115, Calabar,
Nigeria.
Dr. (Mrs.) Nsikan S.
Udoekong
Department of Science and
Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria.
Miss Hannah A. Etuk
Department of Microbiology,
Faculty of Biological Sciences, University of Calabar, P.M.B. 1115, Calabar,
Nigeria.
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