Skip to main content

Inhibition of Nigerian Echis ocellatus Phospholipase A2: Perspectives and Opportunities in Venomics | Chapter 01 | Recent Advances in Biological Research Vol. 4

The most effective and acceptable therapy for snakebite victims is the immediate administration of antivenin following envenomation which is limited by problems of hypersensitivity reactions in sensitive individuals and its inability to resolve the local effects of the venom. Phospholipase A2 Inhibitor from Echis ocellatus Serum (PIES) was isolated, partially purified and characterized. The neutralizing protein from E. ocellatus serum inhibited the E. ocellatus (carpet viper) venom phospholipase A2 (PLA2) enzyme in a dose dependent manner. A two step purification process on sephadex G-200 column chromatography and DEAE- cellulose chromatography gave an active fraction that inhibited the venom PLA2 by 78%. The result from SDS-PAGE showed the inhibitor to be a 24.98kDa protein and its kinetic study revealed a mixed pattern of inhibition on the carpet viper venom PLA2 with an estimated Ki values of 3.8%(v/v) to 7.3%(v/v). The study was carried out at the Department of Biochemistry, Faculty of Science, Ahmadu Bello University Zaria, Nigeria from June 2011 to August 2012. The relevance of these findings towards understanding the biochemistry of carpet viper envenomation and the development of a novel antivenin drug in future targeting the activity of PIES are discussed.


Author(s) Details

F. A. Adamude
Department of Biochemistry, Federal University, Lafia, Nasarawa State, Nigeria.

J. E. Dingwoke
Department of Biochemistry, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.

N. N. Nwobodo
Department of Pharmacology & Therapeutics, College of Medicine, Enugu State University of Science & Technology, Enugu, Enugu State, Nigeria and Department of Pharmacology & Therapeutics, College of Health Sciences, Nile University of Nigeria, FCT, Abuja, Nigeria.

A.Ubhenin
Department of Biochemistry, Federal University, Lafia, Nasarawa State, Nigeria.

View Volume: https://doi.org/10.9734/bpi/rabr/v4

Comments

Popular posts from this blog

Consensus Summit: Lipids and Cardiovascular Health in the Nigerian Population | Chapter 09 | Current Trends in Food Science Vol. 1

Aims: To issue a consensus statement on Lipids and Cardiovascular Health and the impact of their interrelationship in Nigerian Population. Study Design: Experts from a range of relevant disciplines, deliberated on different aspects of Lipids and Cardiovascular Health in the Nigerian Population at a Summit. Place and Duration of Study: The Summit was held in April 2016 at the Nigerian Institute of Medical Research. Methodology: Presentations were made on central themes after which expert participants split into four different groups to consider the questions relevant to different sub themes of the title. Consensus was arrived at, from presentations of groups at plenary. Conclusion: With the increase in the prevalence of NCDs, especially Cardiovascular Disease in Nigeria, and the documented evidence of deleterious effects of lipids, the expert panel called for an urgent need to advocate for the general public and health professionals to make heart-friendly

A Review on Gongronema latifolium, an Extremely Useful Plant with Great Prospects | Chapter 11 | Recent Advances in Biological Research Vol. 3

Gongronema latifolium is a plant that has a wide range of nutritional and ethnomedical uses in different tropical African communities. Scientific reports on the chemical composition and bioactivity (anti-inflammatory, antimicrobial, antidiabetic, antioxidant, anticancer and allelopathic properties) of the plant material by different authors are discussed in this review. Future prospects of the plant extracts in the areas of herbal formulations, food preservation, alcoholic fermentation and beer production, drug discovery and allelopathy are also highlighted. Author   Details: Olugbenga Morebise Faculty of Basic Medical Sciences, All Saints University School of Medicine, Roseau, Commonwealth of Dominica. Read full article: http://bp.bookpi.org/index.php/bpi/catalog/view/50/403/433-1 View Volume: https://doi.org/10.9734/bpi/rabr/v3

An Overview of Ultrasonic Technology and Its Applications in Food Processing, Preservation and Quality Control | Chapter 03 | Current Research in Science and Technology Vol. 3

Ultrasound is one of the emerging technologies that were developed to minimize processing, maximize quality and ensure the safety of food products. In recent years, ultrasound technology has been used as an alternative processing option to conventional thermal approaches. Although Ultrasonication methods have been used for years in research and diagnostics, major advances have been made in the last decade. The applications for which high power ultrasound can be used range from existing processes that are enhanced by the retrofitting of high power ultrasonic technology, to the development of processes up to now not possible with conventional energy sources. The present paper reviews the generation, principle mechanism, properties, process parameters, applications, merits and demerits and future trends of the ultrasound technology in the food processing. Author(s) Details Er. Bogala Madhu Department of Processing and Food Engineering, College of Technology and Engineering, MP