Selection and Characterization of Phosphate Solubilizing Bacteria Isolated from the Rhizosphere of Three Cultivated Legumes in Morocco | Chapter 08 | Advances and Trends in Biotechnology and Genetics Vol. 1
Phosphorus is the most important
nutrient for the growth and development of plants. Phosphate-solubilizing
bacteria (PSB) are known to influence plant growth by enhancing the
availability of soluble P. This study aimed to isolate PSB from the rhizosphere
of three cultivated legumes (fava bean, chickpea, and green peas) and evaluate
their plant growth promotion (PGP) traits. The capacity of isolates to
solubilize unavailable phosphate was quantitatively evaluated in both agar
plate and broth assays using National Botanical Research Institute's phosphate
(NBRIP) medium. One hundred twenty-seven (127) PSBs were isolated from fifteen
fields at different locations in Meknes, and according to their phosphate
solubilization activity in vitro, eight of these isolates showing the greatest
solubilization activity were selected for 16S rDNA sequencing. The selected
isolates were further tested for other PGP traits, such as N2 fixation
activity, indole-3-acetic acid (IAA) biosynthesis, and siderophore production.
P solubilization index (SI) of selected isolates varied from 2.97 to 4.5 and
amount of solubilized phosphorus ranged from 50.95 to 97.49 µg mL-1. All eight
isolates showed the potential to produce IAA, and only two strains T4 and T13
showed positive N2-fixation activity. Also, all selected strains were able to
produce siderophores, except two (T4 and T13). The selected PSBs were
tentatively identified as belonging to seven genera, Rhizobium,
Paraburkholderia, Bacillus, Pantoea, Rahnella, Klebsiella and Enterobacter.
This research extends the knowledge on phosphate solubilizing bacteria in the
rhizosphere of three cultivated legumes from Meknes region and our selected
strains may be used as efficient bio-inoculants in the field to decrease
environmental pollution and promoting sustainable agriculture.
Author(s) Details
Abderrazak Rfaki
Soil and Environment
Microbiology Unit, Faculty of Sciences, Moulay Ismail University, 11201 Meknes,
Morocco.
National Centre for
Scientific and Technical Research (CNRST), 10102 Rabat, Morocco.
Laila Nassiri
Soil and Environment
Microbiology Unit, Faculty of Sciences, Moulay Ismail University, 11201 Meknes,
Morocco.
Professor Jamal Ibijbijen
Soil and Environment
Microbiology Unit, Faculty of Sciences, Moulay Ismail University, 11201 Meknes,
Morocco.
View Volume: https://doi.org/10.9734/bpi/atbg/v1
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