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Selection and Characterization of Phosphate Solubilizing Bacteria Isolated from the Rhizosphere of Three Cultivated Legumes in Morocco | Chapter 08 | Advances and Trends in Biotechnology and Genetics Vol. 1

Phosphorus is the most important nutrient for the growth and development of plants. Phosphate-solubilizing bacteria (PSB) are known to influence plant growth by enhancing the availability of soluble P. This study aimed to isolate PSB from the rhizosphere of three cultivated legumes (fava bean, chickpea, and green peas) and evaluate their plant growth promotion (PGP) traits. The capacity of isolates to solubilize unavailable phosphate was quantitatively evaluated in both agar plate and broth assays using National Botanical Research Institute's phosphate (NBRIP) medium. One hundred twenty-seven (127) PSBs were isolated from fifteen fields at different locations in Meknes, and according to their phosphate solubilization activity in vitro, eight of these isolates showing the greatest solubilization activity were selected for 16S rDNA sequencing. The selected isolates were further tested for other PGP traits, such as N2 fixation activity, indole-3-acetic acid (IAA) biosynthesis, and siderophore production. P solubilization index (SI) of selected isolates varied from 2.97 to 4.5 and amount of solubilized phosphorus ranged from 50.95 to 97.49 µg mL-1. All eight isolates showed the potential to produce IAA, and only two strains T4 and T13 showed positive N2-fixation activity. Also, all selected strains were able to produce siderophores, except two (T4 and T13). The selected PSBs were tentatively identified as belonging to seven genera, Rhizobium, Paraburkholderia, Bacillus, Pantoea, Rahnella, Klebsiella and Enterobacter. This research extends the knowledge on phosphate solubilizing bacteria in the rhizosphere of three cultivated legumes from Meknes region and our selected strains may be used as efficient bio-inoculants in the field to decrease environmental pollution and promoting sustainable agriculture.

Author(s) Details

Abderrazak Rfaki
Soil and Environment Microbiology Unit, Faculty of Sciences, Moulay Ismail University, 11201 Meknes, Morocco.
National Centre for Scientific and Technical Research (CNRST), 10102 Rabat, Morocco.

Laila Nassiri
Soil and Environment Microbiology Unit, Faculty of Sciences, Moulay Ismail University, 11201 Meknes, Morocco.

Professor Jamal Ibijbijen
Soil and Environment Microbiology Unit, Faculty of Sciences, Moulay Ismail University, 11201 Meknes, Morocco.


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